一枝蒿不同溶剂提取物抗病毒作用的谱效关系研究  

Study on the Spectrum-effect Relationship of Anti-virus Effect of Artemisia rupestris Extracts from Different Solvents

作　　者：秦子茹 贺金华 顾政一 沙先谊[3] 

机构地区：[1]新疆医科大学药学院药剂教研室,乌鲁木齐830054 [2]新疆药物研究所,乌鲁木齐830004 [3]复旦大学药学院药剂教研室,上海201203

出　　处：《中国药房》2015年第7期889-893,共5页China Pharmacy

基　　金：国家自然科学基金资助项目(No.U1303224)

摘　　要：目的:研究一枝蒿不同溶剂提取物抗病毒作用的谱效关系。方法:采用高效液相色谱(HPLC)法构建一枝蒿不同溶剂提取物的指纹图谱;进行体外抗流感病毒、乙肝病毒试验,比较其生理活性的差异;采用灰色关联度分析其谱效关系。结果:在构建的一枝蒿不同溶剂提取物的HPLC指纹图谱中确定了9个共有特征峰;各特征峰所代表的各化学成分(以特征峰编号作为该特征峰所代表的化学成分名称)在抗流感病毒中作用的大小顺序依次为9>4>8>6>1>3>7>2>5;在抗乙肝病毒中作用大小顺序依次为7>9>5>8>4>2>1>6>3。结论:一枝蒿不同溶剂提取物的HPLC指纹图谱具有明显的差异,其抗病毒活性与指纹图谱具有一定的相关性。OBJECTIVE:To study the spectrum-effect relationship of anti-virus effect of Artemisia rupestris extracts from different solvents.METHODS:The fingerprint chromatogram of A.rupestris extracts from different solvents was constructed by HPLC.The differences of physiological activity were compared by anti-influenza virus and anti-hepatitis B virus tests in vitro.The spectrum-effect relationship was analyzed using gray relational grades.RESULTS:Nine characteristic peaks were determined in the HPLC fingerprint chromatogram of A.rupestris extracts from different solvents.The represented chemical composition of each characteristic peak(characteristic peak number as the name of the chemical composition of this characteristic peak)on the contribution of different peak to inhibit the influenza virus was ranked with 9>4>8>6>1>3>7>2>5;the contribution of different peak to inhibit the hepatitis B virus was ranked with 7>9>5>8>4>2>1>6>3.CONCLUSIONS:There are significant differences in HPLC fingerprint chromatogram of A.rupestris extracts from different solvents,and their antiviral activity are correlated with fingerprint chromatogram.

关 键 词：一枝蒿 提取物 高效液相色谱法 指纹图谱 谱效关系 

分 类 号：R284.1[医药卫生—中药学]