HPLC法测定氯霉素滴眼液在兔眼房水中的质量浓度  

Concentration Determination of Chloramphenicol Eye Drops in Aqueous Humor of Rabbits by HPLC

作　　者：聂绩 张潜 余凤玮 袁野 

机构地区：[1]重庆市渝北区人民医院,重庆400112

出　　处：《中国药房》2015年第7期921-923,共3页China Pharmacy

摘　　要：目的：测定氯霉素滴眼液在兔眼房水中的质量浓度。方法：在6只兔眼结膜囊内滴入氯霉素滴眼液100μl，于滴眼后5、15、30、60、120、180min取眼房水，加甲醇后涡旋、离心，高效液相色谱法测定上清液中氯霉素质量浓度。色谱柱为Hypersil ODS C18，流动相为甲醇．0．34％磷酸二氢钾水溶液（55：45），检测波长为273nm，流速为1．0ml／min，柱温为30℃，进样量为20μl。结果：氯霉素检测质量浓度的线性范围为0．125-4μg／ml（r=0．9986，n=6），方法回收率为91．28％～98．35％、提取回收率为88．89％～96．92％（n=3），日内RSD为2．98％、日间RSD为3．34％（n=5）；各时间点眼房水样品中氯霉素质量浓度分别为0、（O．331±O．041）、（0．251±O．049）、（0．133±0．052）、（O．068±0．042）、（O．035±0．043）μg／ml。结论：本方法灵敏、结果准确，可用于兔眼房水中氯霉素质量浓度的测定。OBJECTIVE： To establish a method for content determination of Chloramphenicol eye drops in aqueous humor of rabbits. METHODS： 6 rabbits were given Chloramphenicol eye drops 100μl via conjunctival sac; 5, 15, 30, 60, 120 and 180 min later, the aqueous humor was collected, and whirled and centrifuged with methanol. The concentration of chloramphenicol in supernatant was determined by HPLC. The determination was performed on Hypersil ODS C18 column with mobile phase consisted of methanol-0.34% KH2PO4 （55：45） at the flow rate of 1.0 ml/min. The detection wavelength was set at 273 nm and column tem- perature was 30 ℃. The injection volume was 20 μl. RESULTS： The linear range of chloramphenicol was 0.125-4μg/ml （r： 0.998 6, n=6） with method recovery rate of 91.28%-98.35% and extraction recovery rate of 88.89%-96.92% （n=3）. RSDs of in- ter-day and intra-day were 2.98% and 3.34% （n=5）. The concentrations of chloramphenicol were 0, （0.331 ± 0.041）, （0.251 ± 0.049）, （0.133 ± 0.052）, （0.068 ± 0.042） and （0.035 ± 0.043） μg/ml at different time points. CONCLUSIONS： The method is sensi- tive and accurate, and can be used for the determination of chloramphenicol in aqueous humor of rabbits.

关 键 词：高效液相色谱法 氯霉素滴眼液 兔 房水 质量浓度测定 

分 类 号：R927.2[医药卫生—药学] R978.13