大豆籽粒维生素E含量的QTL分析  

Identification of QTL Associated with Vitamin E Content in Soybean Seeds

作　　者：张红梅[1] 李海朝[2] 文自翔[2] 顾和平[1] 袁星星[1] 陈华涛[1] 崔晓艳[1] 陈新[1] 卢为国[2] 

机构地区：[1]江苏省农业科学院蔬菜研究所,江苏南京210014 [2]河南省农业科学院经济作物研究所/国家大豆改良中心郑州分中心/农业部黄淮海油料作物遗传育种重点实验室,河南郑州450002

出　　处：《作物学报》2015年第2期187-196,共10页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(30771360);江苏省农业自主创新基金项目[cx(13)4070];江苏省科技支撑计划项目(BE2013379)资助

摘　　要：维生素E（VE）具有提高人体免疫力、抗癌、预防心血管疾病等保健作用,从大豆中提取的VE安全性更高。本研究采用高效液相色谱技术（HPLC）检测大豆BIEX群体（Essex×ZDD2315）维生素E的α-生育酚、γ-生育酚和δ-生育酚含量。应用QTLNetwork 2.1软件分别检测到8个和12对控制大豆维生素E及组分含量的加性和互作QTL。α-生育酚含量加性和互作QTL累计贡献值分别为8.68%（2个）和15.57%（4对）,γ-生育酚含量加性和互作QTL累计贡献值分别为8.59%（2个）和11.57%（2对）,δ-生育酚含量加性和互作QTL累计贡献值分别为5.44%（1个）和17.61%（3对）,维生素E总含量的加性和互作QTL累计贡献值分别为11.39%（3个）和9.48%（3对）。未检测到维生素E及组分含量和环境互作的QTL。未定位到的微效QTL累计贡献值为66.16%-75.32%,说明未定位到的微效基因的变异占2/3以上。各性状的遗传构成中,未检测出的微效QTL份额最大,加性QTL和互作QTL贡献相差不大。在育种中应考虑常规方法聚合微效QTL与标记辅助方法聚合主要QTL相结合。Vitamin E has effects on human immunity, anti-cancer and prevention of cardiovascular disease. Vitamin E from soy-bean has the advantages of higher security and higher human body absorption rate. The objective of the present study was to map the additive, additive × additive （epistasis）, additive ＆#215; year and epistasis × year QTLs for vitamin E and relative tocopherol con-tents with the RIL population BIEX （Essex×ZDD2315） using HPLC （high performance liquid chromatography） method and soft-ware QTLNetwork 2.1. Eight additive QTLs and twelve additive ＆#215; additive （epistasis） QTLs were detected for vitamin E and relative tocopherol contents. The contributions to the phenotypic variances of additive QTL and epistatic QTL pairs were 8.68%（two QTLs） and 15.57% （four pairs） for α-tocopherol, 8.59% （two QTL） and 11.57% （two pairs） for γ-tocopherol, 5.44% （one QTL） and 17.61% （three pairs） for δ-tocopherol and 11.39% （three QTL） and 9.48% （three pairs） for total vitamin E contents, respectively. Those of additive and epistatic QTLs by year interaction were not found. The accumulated contribution of the un-mapped minor QTLs was 66.16%–75.32%, indicating the variance of unmapped minor QTLs accounting for more than two thirds. In genetic composition, undetected minor QTLs accounted for a considerably large part additive QTLs and epistatic QTLs were nearly equal in α-tocopherol, γ-tocopherol, δ-tocopherol and total vitamin E contents. Accordingly, in breeding for vitamin E con-tents, the strategy of pyramiding multiple QTLs, both additive and epistatic, by using marker-assisted selection combined with accumulating minor effect QTLs through conversional procedures should be considered.

关 键 词：大豆 籽粒 维生素E含量 QTL定位 

分 类 号：S565.1[农业科学—作物学]