小麦–华山新麦草异代换系DH2322的分子细胞遗传学鉴定  

Molecular Cytogenetics Identification of a wheat Psathyrostachys huashanica Substitution Line DH2322

作　　者：王秀娟[1] 陈新宏[1] 庞玉辉[1] 敬樊 张军[1] 胡思远[1] 昝凯 武军[1] 杨群慧[1] 赵继新[1] 

机构地区：[1]西北农林科技大学农学院,陕西杨凌712100

出　　处：《作物学报》2015年第2期207-213,共7页Acta Agronomica Sinica

基　　金：国家高技术研究发展计划(863计划)项目(2011AA100501);陕西省自然科学基础研究计划重点项目(2013JZ007);中央高校基本科研业务费(QN2011001);西北农林科技大学唐仲英育种基金资助

摘　　要：利用分子标记、细胞学、基因组原位杂交(GISH)等技术,结合田间农艺性状调查,对小麦–华山新麦草七倍体材料H8911与硬粒小麦D4286杂交F4代分离群体中株系DH2322进行了综合鉴定。华山新麦草基因组特异SCAR标记鉴定表明,DH2322含有华山新麦草遗传物质;细胞遗传学观察显示,DH2322染色体构型为2n=42=21 II;有丝分裂和花粉母细胞减数分裂中期I基因组原位杂交(GISH)鉴定表明,DH2322的染色体由40条小麦染色体和2条华山新麦草Ns染色体构成,且2条Ns染色体能完全配对为一个二价体;SSR和STS分子标记分析表明,DH2322缺失小麦D基因组的2D染色体,而含有华山新麦草的2Ns染色体;农艺性状分析结果表明,DH2322具有双亲的形态学特征,结实性好,穗长和穗粒数显著大于亲本。A wheat–P. huashanica alien substitution line DH2322 was isolated from the F4 progeny of heptaploid hybrid H8911 ＆#215; Triticum durum cv. D4286 and identified by using SCAR markers, genomic in situ hybridization （GISH） , SSR markers, and STS markers. The results using SCAR marker RHS141 showed that DH2322 carrys chromosome derived from P. huashanica. Cyto-genetics observation indicated that DH2322 has a chromosome karyotype of 2n = 42 = 21 II. GISH showed that DH2322 is a line with 40 chromosomes from wheat and two Ns chromosomes from P. huashanica, which formed a ring bivalent at metaphase I in pollen mother cells （PMC）. According to SSR and STS analysis, the wheat chromosome 2D in DH2322 was substituted by the chromosome 2Ns from P. huashanica. Evaluation of the agronomic traits showed that DH2322 presented favorable agronomic traits in spike length and kernels per spike. Development of this new germplasm will be useful for wheat breeding programs in the future.

关 键 词：普通小麦 华山新麦草 异代换系 SCAR 基因组原位杂交(GISH) SSR STS 

分 类 号：S512.1[农业科学—作物学]