贝那普利对高血压患者内皮祖细胞凋亡及氧化应激的影响  

Effects of Benner Pury on endothelial progenitor cells apoptosis and oxidative stress in patients with hypertension

作　　者：李永东[1,2] 葛智平[1] 温慧华[1] 刘丹[1] 阿拉坦高勒[2] 

机构地区：[1]内蒙古医科大学第三附属医院心内科,包头014010 [2]内蒙古大学生命科学学院

出　　处：《中国心血管杂志》2015年第1期52-56,共5页Chinese Journal of Cardiovascular Medicine

基　　金：内蒙古自治区自然科学基金项目(2011MS1150)~~

摘　　要：目的探讨贝那普利对高血压患者内皮祖细胞(EPC)凋亡和氧化应激的影响。方法入选符合《中国高血压防治指南》诊断标准的1级高血压患者15例,同时选取年龄、性别相匹配的健康体检者15名为对照组。所有受试者抽取外周血,常规密度梯度离心法分离单个核细胞,将经鉴定的正在分化的EPC培养5 d后用于实验。将分离、培养获得的每一例高血压患者EPC培养5 d后将细胞平行分为5份,每份细胞根据不同的刺激处理条件分为高血压组、不同浓度贝那普利(1、10和100μmol/L)干预组、贝那普利(10μmol/L)+基质细胞衍生因子/受体趋化因子4(SDF-1/CXCR4)拮抗剂AMD3100干预组。上述干预24 h后进行EPC凋亡检测:用Annexin-V/PI法;氧化应激检测:黄嘌呤氧化法测定上清液中超氧化物歧化酶活力,硫代巴比妥酸法测定丙二醛含量。结果 (1)与对照组比较,高血压组外周血EPC凋亡率明显增加(t早=6.6334,t晚=3.812;均为P<0.01),氧化应激反应明显增高(tSOD=8.258,tMDA=6.6806;均为P<0.01);(2)随着贝那普利干预浓度的增加(1、10和100μmol/L),EPC凋亡率也随之降低(F早=17.39,F晚=51.65;均为P<0.05),氧化应激反应也随之降低,呈浓度依赖性(FSOD=14.56,FMDA=7.859;均为P<0.05)。与10μmol/L贝那普利干预组比较,10μmol/L贝那普利+AMD 3100干预组的EPC凋亡率增加(t早=3.551,t晚=5.333;均为P早、晚<0.05),氧化应激反应增高(tSOD=1.931,tMDA=0.6407;均为P<0.05)。结论贝那普利能显著改善高血压患者体外EPC凋亡及氧化应激反应,并可能通过SDF-1/CXCR4轴发挥作用。Objective To investigate the effection of Benner Pury on endothelial progenitor cells apoptosis and oxidative stress in vitro hypertension. Methods The patients who were diagnosed as hypertension grade 1, according to the standard ″Guidelines for Prevention and Treatment of Hypertension″. The control group were healthy subjects matched with study group in age and gender. Mononuclear cells were isolated by density gradient centrifugation, and fluorescence chemical staining in acLDL-DiI, FITC-lectin, observed under confocal laser scanning microscope, staining positive cells were considered as the differentiation of endothelial progenitor cell（EPC）. EPC cultivated for 5 days were used for study. The cells isolated from each hypertensive were divided into five copies in parallel, and according to the different stimulation, the cells were divided into five groups： hypertension group, different concentrations of Benner Pury （1 umol/L, 10 umol/L, 100 umol/L） intervention in hypertension group, and Benner Pury （10 umol/L） ＋stromal cell derived factor-1（SDF-1）/its receptor chemokine 4 （CXCR4） antagonist AMD 3100 pretreatment group. EPC cultivated for 24 h under the different stimulation were used for study EPC apoptosis detection：Annexin-V/PI method; oxidative stress detection： the activity of superoxide dismutase （ SOD） , xanthine-oxidation method, the content of Malonaldehyde （ MDA） malondialdehyde thiobarbituric acid method. Results （ 1 ） Compared with the control group, peripheral blood EPS apoptosis rate was obviously increased in hypertension group, oxidative stress was significantly increased too （ tearly =6. 6334, tlate =3. 812; both p〈0. 01）. （2） Detection of the apoptosis and oxidative stress level display, different concentrations of Benner Pury 1 umol/L, 10 umol/L, 100 umol/L intervention in vitro hypertensive patients EPC for 24 h, with the concentrations of Benner Pury gradually increased, the apoptosis rate of EPC was reduced （Fearly =17. 39, Flate =51

关 键 词：高血压 内皮祖细胞 凋亡 氧化应激 拮抗剂AMD 3100 

分 类 号：R544.1[医药卫生—心血管疾病]