质谱技术在临床微生物实验室中的应用前景  

The prospects of mass spectrometry application on microbiology

作　　者：罗燕萍[1] 

机构地区：[1]解放军总医院微生物科,北京100853

出　　处：《检验医学》2015年第2期97-101,共5页Laboratory Medicine

摘　　要：目前应用于临床微生物实验室的基质辅助激光解析电离飞行时间质谱(MALDI-TOF MS)是一种直接从完整细菌中获得蛋白指纹图谱,从而对细菌进行快速鉴定的方法,由于可以对常见细菌,诺卡菌、分枝杆菌、厌氧菌、酵母菌及丝状真菌进行快速和准确的鉴定,近年来备受推崇。该文对这次质谱专题刊登的1篇综述和4篇论著进行总结评价。相信随着相关研究的不断深入,这项技术在临床微生物实验室将有更广阔的应用空间。The matrix-assisted laser desorption / ionization-time of flight mass spectrometry（ MALDI-TOF MS）technique can be used to generate protein fingerprint signatures from whole bacterial cells. By comparing these fingerprints to a database of reference spectra by the various algorithms,bacteria can be rapidly identified. MALDI-TOF MS can be used for accurate and rapid identification of various microorganisms,such as common bacteria,Nocardia,Mycobacterium,anaerobe,yeast and filamentous fungi. MALDI-TOF MS have recently been widely introduced. The characteristics of one review and four papers published in the special subject about MALDI-TOF MS were reviewed. The development of studies in MALDI-TOF MS will play an important role in the application of mass spectrometry in clinical microbiological laboratories.

关 键 词：基质辅助激光解析电离飞行时间质谱 细菌鉴定 临床微生物实验室 

分 类 号：R446.5[医药卫生—诊断学]