慢性肾小球肾炎患者外周血Th1/Th2及CD28^+细胞的变化及其临床意义  

The variation and its clinical significance of Th1/Th2 and CD28^+ cells from peripheral blood in patients with chronic glomerulonephritis

作　　者：周韵娇[1] 李鹏[2] 张丽红[2] 钱英俊[2] 牛建英[2] 

机构地区：[1]复旦大学附属上海市第五人民医院中心实验室,上海200240 [2]复旦大学附属上海市第五人民医院肾内科,上海200240

出　　处：《检验医学》2015年第2期141-144,共4页Laboratory Medicine

基　　金：闵行区科委自然基金项目(2010MHZ027)

摘　　要：目的探讨慢性肾小球肾炎(CGN)患者外周血Th1/Th2及CD28+细胞百分率的变化在疾病进程中可能的细胞免疫学机制。方法采用流式细胞术检测50例CGN患者及30名正常人(对照组)外周血Th1/Th2和CD28+细胞的百分率,用SPSS 16.0软件对试验结果进行统计分析。结果与对照组比较,CGN患者外周血CD3+CD4+细胞百分率和CD4/CD8比值明显降低(P<0.05),Th1和Th2细胞百分率差异无统计学意义(P>0.05),但Th1/Th2比值明显增高(P<0.05);CGN患者CD28+、CD4+CD28+细胞百分率明显减少(P均<0.01)。结论 CGN患者体内T细胞免疫功能紊乱和活化受限,细胞免疫功能低下,Th1和Th2细胞失衡,通过对CGN患者外周血Th1/Th2及CD28+细胞的检测,有助于了解患者的细胞免疫状态,为临床诊断和免疫治疗提供参考依据。Objective To investigate the possible mechanism of cellular immunology in the process of chronic glomerulonephritis（ CGN） by the detection of Th1 / Th2 ratio and CD28^＋cell percentages from peripheral blood in patients with CGN. Methods Th1 / Th2 ratio and CD28^＋cell percentages were detected by flow cytometry from 50 patients with CGN and 30 healthy subjects（ control group）. SPSS 16. 0 software was used to analyze the results.Results Compared with control group,the percentages of CD3^＋CD4^＋cells and CD4 / CD8 ratio of patients with CGN decreased significantly（ P〈0. 05）. Although there was no statistical significance in the percentages of Th1 and Th2 cells（ P〉0. 05）,yet Th1 / Th2 ratio increased significantly（ P〈0. 05）. The percentages of CD28^＋and CD4^＋CD28^＋cells decreased significantly（ P〈0. 01）. Conclusions T cell immune function is disorder,and activation is restricted in patients with CGN. Their cellular immune function is low,and there exists imbalance between Th1 and Th2 cells.The detection of Th1 / Th2 ratio and percentages of CD28^＋cells from peripheral blood in patients with CGN is valuable to understand the immune state so as to provide the theoretical basis for immune therapy.

关 键 词：TH1/TH2细胞 CD28^+细胞 流式细胞术 慢性肾小球肾炎 

分 类 号：R446.63[医药卫生—诊断学]