耐甲氧西林金黄色葡萄球菌MecA基因的实时荧光定量PCR方法学评价  

Evaluation of FQ-PCR method for detecting methicillin-resistant Staphylococcus aureus MecA gene

作　　者：欧红玲[1] 陈凤华[1] 王岩[1] 张巧云[1] 王欣茹[1] 

机构地区：[1]第二炮兵总医院检验科,北京100088

出　　处：《检验医学与临床》2015年第4期436-438,共3页Laboratory Medicine and Clinic

基　　金：全军十二五面上课题(CWS11J205)

摘　　要：目的 评估实时荧光定量聚合酶链反应（FQ-PCR）检测临床标本中耐甲氧西林金黄色葡萄球菌（MRSA）MecA耐药基因的价值。方法 选择该院2013年6~12月125份临床标本,包括血液40份,痰液52份,创面分泌物33份进行FQ-PCR检测和细菌鉴定,分析结果符合率,以检测FQ-PCR的灵敏度及特异性。结果 FQ-PCR的灵敏度为0.159pg/μL,检测经细菌培养鉴定的菌株,特异性达到100%,与临床标本结果符合率为97.6%。结论 FQ-PCR检测MRSA的MecA基因更加方便、快速,且灵敏度和特异性等性能指标均比较理想,与细菌鉴定结果符合率较高,适合临床广泛应用。Objective To evaluate the reliability of fluorescent quantitative polymerase chain reaction（FQPCR）method for detecting methicillin-resistant Staphylococcus aureus（MRSA）MecA resistant gene.Methods A total of 125 clinical specimens were collected from June 2013 to December 2013,including 40 blood specimens,52 sputum specimens,33 specimens of wound secretions.FQ-PCR method and bacteria identification were used to detect all the specimens,and then analyze the consistent rate of results,sensitivity and specifity.Results The sensitivity of FQ-PCR method was 0.159pg/μL,the specificity of FQ-PCR in detecting bacterial strain cultured and identified was100%,and the consistent rate with the results of clinical samples was 97.6%.Conclusion FQ-PCR method is more convenient,fast in detecting MecA gene of MRSA,and sensitivity,specificity are ideal,the consistent rate with bacterial identification result is high,so could be used widely in clinical applications.

关 键 词：耐甲氧西林金黄色葡萄球菌 MECA基因 实时荧光定量聚合酶链反应 微生物鉴定 

分 类 号：R378.11[医药卫生—病原生物学]