牛、羊布鲁氏菌病试管凝集试验与微量凝集试验检测方法的比较  

Comparison of serum tube agglutination test and micro agglutination test on brucellosis detection in cattle, goat and sheep

作　　者：赵凤菊[1] 李璐[1] 闫明媚[1] 于学武[1] 陈瑶[1] 李井春[1] 

机构地区：[1]辽宁省动物疫病预防控制中心,辽宁沈阳110164

出　　处：《现代畜牧兽医》2015年第2期8-10,共3页Modern Journal of Animal Husbandry and Veterinary Medicine

基　　金：辽宁省农业攻关支撑计划;编号:201404210

摘　　要：血清学检测方法因其快速、安全、高效等特点被广泛应用于布鲁氏菌病的检测和诊断。本文通过对保存的226份牛、羊血清样品同时使用试管凝集法与微量凝集试验进行检测并比较分析其在诊断中的意义。结果显示,微量法与试管法两者的符合率为97.79%,两种检测方法无统计学差异。但微量法的阳性检出率要高于试管法,同时由于微量法具有操作简便、快捷及结果判定更为直观、简单等优点,因此,微量凝集试验更适宜在我国基层布鲁氏菌病大量样本监测、诊断中推广应用。Serologic tests were widely used in the detection and diagnosis of brucellosis, because of its fast, safe, efficient, and other characteristics. In order to compare and analyze its significance in diagnosis, a total of 226 cattle, goat, and sheep serum samples were detected by both tube agglutination and micro agglutination test at the same time in this study. The results showed that the coincidence rate of the tube agglutination test and micro agglutination test was 97.79%; and there was no significant difference in two methods. However, the positive rate of micro agglutination test was higher than tube agglutination test. Micro agglutination test was more suitable for brucellosis surveillance and diagnosis, for it was a simple, fast and more intuitive diagnosis method.

关 键 词：布鲁氏菌病 试管凝集试验 微量凝集试验 

分 类 号：S858.23[农业科学—临床兽医学]