单纯疱疹病毒1型(HSV-1)糖蛋白B基因疫苗对HSV-1原发及潜伏感染的抑制作用  

Inhibitory effect of herpes simplex virus type 1 glycoprotein B DNA vaccine on primary and latent infection with the virus

作　　者：孟祥俊[1] 苏云[1] 

机构地区：[1]大连大学附属中山医院,辽宁大连116001

出　　处：《中国生物制品学杂志》2015年第1期17-20,共4页Chinese Journal of Biologicals

基　　金：大连市卫生局资助课题(大卫科发:[2009]67号)

摘　　要：目的观察单纯疱疹病毒1型（herpes simplex virus-1,HSV-1）糖蛋白B基因疫苗（pg B）在防治HSV-1原发及潜伏感染的作用。方法将BALB/c小鼠随机分为4组：A组（实验组,注射含质粒pg B的PBS）、B组（阳性对照组,注射灭活的HSV-1病毒液）、C组（载体对照组,注射含质粒pc DNA3的PBS）和D组（空白对照组,注射PBS）。各组小鼠均于双侧股四头肌多点注射,共免疫3次,间隔3周。于末次免疫3周后行角膜划痕接种HSV-1。术后1～14、21和28 d行角膜荧光素染色,在裂隙灯显微镜下观察角膜炎病变形成情况。角膜接种病毒第30天,处死全部小鼠,取三叉神经节,接种Vero细胞,观察病毒潜伏感染形成情况。结果 A组和B组小鼠接种HSV-1后仅有轻度角膜上皮炎发生,且6 d内全部愈合,组间比较差异无统计学意义（P〉0.05）;C组和D组小鼠接种后第2天角膜上皮炎达到高峰,7 d后炎症慢慢消失,与A组比较,差异有统计学意义（P〈0.05）。A组和B组小鼠接种后未发生角膜基质炎,且无1例死亡;C组和D组小鼠在接种后第5天出现炎症反应,并逐渐加重,接种后11～14 d角膜基质炎达到高峰,在接种后第30天有30%小鼠死亡。Vero细胞检测病毒潜伏感染,A组和B组未发生细胞病变,而C组和D组第5天发生细胞病变。结论 pg B能有效预防实验小鼠HSV-1原发感染,并抑制潜伏感染的形成。Objective To observe the role of herpes simplex virus type 1 （HSV-1） glycoprotein B （pgB） DNA vaccine in the primary and latent HSV-1 infections. Methods BALB/c mice were randomly divided into test （A）, positive control （B）, vector control （C） and blank control （D） groups. The mice in group A were immunized i.m. with PBS containing plasmid pgB, while those in group B with inactivated HSV-1, those in group C with PBS containing plasmid pcDNA3, and those in group D with PBS. The mice in various groups were immunized for 3 times each at an interval of 3 weeks, and inoculated with HSV-1 by scarification in cornea 3 weeks after the last immunization. All the mice were killed on day 30 after inoculation, of which intrigeminal ganglia was inoculated to Vero cells, and the latent HSV-1 infection was observed. Results Only mild corneal epithelitis was observed in the mice in groups A and B after inoculation with HSV-1, which was recovered within 6 d and showed no significant difference in various groups （P 〉 0. 05）. The corneal epithelitis was the most serious on the 2nd day in groups C and D, which disappeared gradually 7 d later and showed significant difference with that in group A （P 〈 0. 05）. No interstitial keratitis or death was observed in groups A and B. However, inflammatory reactions were observed in groups C and D on day 5 after inoculation, which was exacerbated gradually and was most serious on day 11 - 14 after inoculation, while 30% of mice died on day 30. Latent HSV-1 infection was observed in Vero cells. No CPEs were observed in groups A and B, while were observed on day 5 after inoculation in groups C and D. Conclusion HSV-1 pgB effectively prevented primary infection and inhibited the latent infection with HSV-1 in mice.

关 键 词：单纯疱疹病毒1型 糖蛋白B 基因疫苗 原发感染 潜伏感染 单纯疱疹病毒性角膜炎 

分 类 号：R373.11[医药卫生—病原生物学] R392-33[医药卫生—基础医学]