抗-HCV初筛阳性血浆的WB确证检测及核酸检测结果分析  

Analysis on result of confirmation assays and nucleic acid test for plasma samples proved as anti-HCV positive by ELISA

作　　者：张容 邓锷 柯玲 吴秉婷 刘桂 徐敏 刘鱼 李长清 

机构地区：[1]中国医学科学院输血研究所,四川成都610052

出　　处：《中国生物制品学杂志》2015年第1期63-67,共5页Chinese Journal of Biologicals

基　　金：国家卫生公益性行业科研专项(201002005)

摘　　要：目的探讨丙型肝炎病毒抗体(抗-HCV)初筛阳性血浆的HCV核酸检测(nucleic acid testing,NAT)及蛋白免疫印迹(Western blot,WB)确证结果的相关性。方法使用1种HCV抗体确证试剂及1种核酸检测试剂,分别检测抗-HCV初筛为阳性的人血浆172份。结果 172份样本中,WB确证及NAT检测结果阳性的样本分别为115和124份,两种试剂检测结果共同阳性的样本100份,共同阴性的样本26份,不一致样本46份。WB确证阳性的样本检测条带强度越强,确证检测及核酸检测共同阳性率越高;确证检测条带数越多,确证检测及核酸检测共同阳性率越高。检测结果不一致的样本中,WB阳性、NAT阴性样本15份;WB阴性、NAT阳性样本16份;WB不确定、NAT阴性样本7份;WB不确定、NAT阳性样本8份。结论抗-HCV初筛阳性样本的WB确证与NAT检测结果既相关又存在一定差异,WB结合NAT检测可更大限度地保障输血安全。Objective To investigate the correlation between the results of confirmation assays （Western blot, WB） and nucleic acid test （NAT） for plasma samples proved as anti-hepatitis C virus （anti-HCV） positive by ELISA. Methods A total of 172 anti-HCV positive serum samples proved by primary screening were retested by one HCV antibody confirmation kit and one NAT kit. Results Of the 172 samples, 115 and 124 were confirmed as positive by WB and NAT respectively, while 100 as positive and 26 as negative by both tests. However, the confirmation results of 46 samples by the two tests were inconsistent. The stronger band intensity was, the higher NAT-positive rate was. Of the 46 samples with inconsistent results, 15 were confirmed as positive by WB and negative by NAT, while 16 were negative by WB and positive by NAT, 7 were indeterminate by WB and negative by NAT, and 8 were indeterminate by WB and positive by NAT. Conclusion There is some correlation and difference in the results of NAT and confirmation assay on plasma samples proved as anti- HCV positive by ELISA. WB and NAT should be used simultaneously in HCV test to ensure the safety of blood.

关 键 词：丙型肝炎病毒 免疫印迹 核酸检测 

分 类 号：R373.21[医药卫生—病原生物学] R446.61[医药卫生—基础医学]