禽I型副粘病毒天鹅源分离株对鸡的致病性研究  

Pathogenicity of an avian paramyxovirus serotype 1 isolate from swan to chickens

作　　者：赵莎莎[1] 韩宗玺[1] 邵昱昊[1] 孔宪刚[1] 刘胜旺[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/禽呼吸道病创新团队,黑龙江哈尔滨150001

出　　处：《中国预防兽医学报》2015年第2期140-142,共3页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家蛋鸡产业技术体系(NYCYTX-41-G10)

摘　　要：为研究禽I型副粘病毒(APMV-1)天鹅分离株sw/CH/LHLJ/120608对鸡的致病性,本研究将该病毒以105EID50/m L的剂量,采用点眼和滴鼻的方式感染SPF鸡,检测其致病性,同时,采用荧光定量RT-PCR的方法检测病毒在各组织中的分布。结果显示,sw/CH/LHLJ/120608分离株感染鸡能够引起30%的发病率和6%的死亡率,并且病毒在感染鸡的呼吸系统、消化系统、免疫系统以及泌尿系统中均有广泛的分布。根据对APMV-1实验动物致病性的判定标准,该病毒株对鸡具有中等毒力的致病力特征。To evaluate the pathogenicity of avian paramyxovirus serotype 1 （APMV-1） to chickens which isolated from swan, the SPF chickens were artificially infected with the APMV-1 at a dose of 10s EID_50/mL via oculonasal administration. The results showed that the isolate caused about 30% morbidity and 6% mortality of the infected chickens. In addition, the virus was distributed widely in respiratory, gastrointestine, immune and urinary system of the chickens detected by real-time PCR. In conclusion, the isolate of APMV-1 was the mesogenic virus to SPF chicken according to the pathogenic criteria.

关 键 词：禽I型副粘病毒 天鹅源 致病力 

分 类 号：S852.65[农业科学—基础兽医学]