模拟航天失重大鼠自发活动的改变  

The different characteristics of spontaneous activity in simulated weightlessness in rats

作　　者：薛涛[1,2] 邬丽莎[1] 陈善广[3] 李莹辉[4] 曲丽娜[4] 刘新民[2] 王琼[1] 

机构地区：[1]泸州医学院,四川泸州646000 [2]中国医学科学院药用植物研究所,北京100093 [3]中国航天员科研训练中心人因工程重点实验室,北京100094 [4]中国航天员科研训练中心航天医学基础与应用国家重点实验室,北京100094

出　　处：《中国比较医学杂志》2015年第1期20-29,共10页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金项目(81001655);航天医学基础与应用国家重点实验室开放基金资助项目(SMFA12K01);人因工程重点实验室开放基金资助项目(HF2012-K-06);全军医药卫生"十二五"科研项目(BWS11J052)

摘　　要：目的通过观察模拟航天失重尾吊21 d大鼠自主活动的改变,为航天失重引起功能改变提供评价方法以及为航天防护措施研究提供参考。方法 30只Wistar大鼠随机分为对照组、假尾吊组、尾吊组,每组10只。将所有动物置于模拟失重尾部悬吊实时监测装置中进行造模21 d,造模期间每周检测动物摄食饮水量和体重变化,同时,每天8:00pm提取当天被监控动物(8:00am^12:00am)、(2:00pm^6:00pm)、白天(8:00am^8:00pm)、晚上(8:00pm^8:00am)、24 h等5个时段自主活动数据。结果正常大鼠上、下午运动量无显著差异,晚上活动量显著高于白天活动量,运动时间和运动路程是白天的2~3倍;尾吊组大鼠在造模10 d后昼夜节律开始紊乱,昼夜运动量越来越接近,假尾吊组大鼠造模10 d内运动状态不稳定,个体差异较大,10 d后趋于稳定,昼夜运动量比值接近对照组大鼠。结论正常大鼠为夜行性动物,晚上活动量为白天活动量的2~3倍,假尾吊组大鼠经21 d造模后昼夜节律不改变,而尾吊21 d后大鼠昼夜节律逐渐消失。Objective To observe the different characteristics of spontaneous activitiy after simulated weightlessness 21 days in rats,aimed to provide a evaluation method for space weightlessness induced function change in human beings and to provide a reference for researches on the astronauts protective measures. Methods 30 Wistar male rats were randomly divided into three groups,the control group,the sham tail-suspended hindlimb unloading group（ thesham group）,the tail-suspended hindlimb unloading group（ the suspending group）,ten animals in each group. All animals were placed in the simulated space flight environmental equipment which has a real-time monitor system for 21 days. During the 21 days,the intake of water,food and the body weight were measured every week. Meanwhile,five independent activity data were collect every day,for example,morning（ 8： 00 am - 12： 00am）,afternoon（ 2： 00 pm - 6：00pm）,daytime（ 8： 00 am - 8： 00pm）,night（ 8： 00 pm - 8： 00am）,and whole day（ 8： 00 am - 8： 00am）. Results The spontaneous activity of normal rats in the control group between morning and afternoon had no significant difference,but it is significantly between night and daytime. The movement time and distance in night are 2- 3 times than that of the daytime. After 10 days of tail suspending,the circadian rhythm was disordered,and the spontaneous activity in day and night become more similar in rats of the suspending group. Because of the individual difference,the spontaneous activity is not stable at the first 10 days in rats of the sham group,but after 10 days,it become close to the control group. Conclusion Rat is nocturnal animal and sleeps in the daytime,the spontaneous activity in night is 2- 3 times as compared with the daytime. The sham tail-suspended hindlimb unloading 21 days can not influence the circadian rhythm in rats. Tail suspending 21 days will caused to the disappearance in the circadian rhythm in rats.

关 键 词：模拟失重 大鼠 自发活动 

分 类 号：Q95-33[生物学—动物学] R332[医药卫生—人体生理学]