缺氧预处理对颅脑损伤大鼠脑组织MMP-9表达及含水量的影响  

Effects of hypoxic preconditioning on expression of MMP-9 and water content in the brain tissue of rats with traumatic brain injury

作　　者：卓健伟 刘家传[1] 杨艳艳[1] 王金标[1] 张星[1] 周治民[1] 孙文江[1] 疏龙飞 

机构地区：[1]安徽医科大学解放军临床学院附属解放军105医院神经外科,合肥230031

出　　处：《中国微侵袭神经外科杂志》2015年第2期87-89,共3页Chinese Journal of Minimally Invasive Neurosurgery

基　　金：全军医学科技"十二五"科研项目(编号:CWS11J262);2009年度南京军区医学科技创新重点课题(编号:09Z009)

摘　　要：目的观察缺氧预处理对颅脑损伤(TBI)大鼠脑组织基质金属蛋白酶-9(MMP-9)的表达和含水量变化。方法 SD雄性大鼠108只,随机分为对照组(n=6)、缺氧预处理组(HPC组,n=6)、TBI组(n=48)、缺氧预处理+外损组(HPCT组,n=48)。采用干湿重法测定脑组织含水量,RT-q PCR、Western blotting分别测定MMP-9 m RNA及蛋白表达水平。结果 TBI组和HPCT组MMP-9 m RNA在伤后3h、6h、12h、1d、3d明显升高(P<0.05),MMP-9蛋白表达和脑组织含水量均在伤后3h、6h、12h、1d、3d、7d明显增加(P<0.05)。HPCT组中MMP-9 m RNA在伤后3h、6h、12 h、1d、3d,MMP-9蛋白和脑组织含水量在伤后6h、12h、1d、3d、7 d明显低于TBI组(P<0.05)。结论缺氧预处理能抑制颅脑损伤脑组织MMP-9表达从而减轻脑水肿,可能是缺氧预处理对颅脑损伤大鼠发挥脑保护作用的机制之一。Objective To investigate the effect of hypoxic preconditioning on expression of MMP-9 and the water content of the brain tissue after traumatic brain injury in rats. Methods One hundred and eight adult male rats were randomly divided into control group（n = 6）, hypoxic preconditioning group（HPC, n = 6）, traumatic brain injury group（TBI, n = 48） and traumatic brain injury after hypoxic preconditioning group（HPCT, n = 48）. The brain water content was measured by dry-wet weight method, and m RNA and protein of MMP-9 was detected by RT-q PCR and Western blotting. Results The m RNA level of MMP-9 in TBI and HPCT groups were markedly increased at 3, 6 and 12 h, and 1 and 3 d（P 0.05）, and the expression of MMP-9 protein and brain water content were significantly higher at 3, 6 and 12 h, and 1, 3 and 7 d（P〈0.05） after injury. The m RNA level of MMP-9 at 3, 6 and 12 h, and 1 and 3 d,and protein of MMP-9 and brain water content at 6 and 12 h, and 1, 3 and 7 d were significantly lower in HPCT than TBI group（P〈0.05）. Conclusions Hypoxic preconditioning can reduce the extent of cerebral edema by inhibiting expression of MMP-9 after traumatic brain injury. This may be one of the mechanisms that hypoxic preconditioning can protect traumatic brain injury in rats.

关 键 词：颅脑损伤 缺氧预处理 基质金属蛋白酶 含水量 

分 类 号：R651.15[医药卫生—外科学]