C57BL/6J小鼠慢性束缚应激抑郁症模型的建立  

Establishment of a Chronic Restraint Stress Model of Depression with C57BL /6J Mice

作　　者：孙楠楠[1,2] 沙龙泽[1,2] 许琪[1,2] 

机构地区：[1]中国医学科学院基础医学研究所 [2]北京协和医学院基础学院生物化学与分子生物学系医学分子生物学国家重点实验室,北京100005

出　　处：《中国医学科学院学报》2015年第1期8-11,共4页Acta Academiae Medicinae Sinicae

基　　金：国家自然科学基金(31222031);中央高校基本科研业务费专项资金(2012S05);协和青年科研基金(2012J09)~~

摘　　要：目的通过慢性束缚应激（CRS）建立C57BL/6J小鼠抑郁症模型。方法 C57BL/6J小鼠经体重、糖水偏好、旷场实验初筛后从中选择20只小鼠并随机分为正常对照（NC）组和CRS组2组,每组10只。对CRS组小鼠进行连续21 d,每天4 h的束缚刺激,并最终进行糖水偏好检测和强迫游泳检测。结果 CRS组小鼠在水中不动的时间为（131.70±21.65）s,明显长于NC组的（68.88±8.43）s（P=0.0304）。CRS组小鼠0～24 h和0～48 h的糖水偏好百分比分别为（66.21±3.24）%和（73.25±1.50）%,均明显低于NC组的（79.46±3.85）%（P=0.0196）和（80.20±2.26）%（P=0.0248）。结论通过慢性束缚应激成功建立C57BL/6J小鼠抑郁症模型。Objective To establish a chronic restraint stress（ CRS） model of depression, using C57 BL /6J mice. Methods Totally 20 C57 BL /6J mice were screened by weight,sucrose preference,and the results of open-field test. Then,they were equally randomized into two groups： normal control（ NC） group and CRS group. Mice in the CRS group were under restraint 4 hours a day and their behavioral changes were evaluated after 21 days. Results The immobility time was significantly longer in CRS mice [（ 131. 70 ± 21. 65） s]compared with controls [（ 68. 88 ± 8. 43） s]（ P = 0. 0304）. CRS mice showed a significant decrease in sucrose preference during the time 0-24 h [（ 66. 21 ± 3. 24） % vs.（ 79. 46 ± 3. 85） %,P = 0. 0196] and 0-48 h[（ 73. 25 ± 1. 50） % vs.（ 80. 20 ± 2. 26） %,P = 0. 0248]compared with controls. Conclusion The C57BL/6J mice CRS models of depression were successfully established.

关 键 词：抑郁症 慢性束缚应激 C57BL/6J小鼠 行为学检测 

分 类 号：R749[医药卫生—神经病学与精神病学]