脂肪间充质干细胞移植治疗兔耳静脉淤血皮瓣的实验研究  

Effect of adipose derived mesenchymal stem cells on the therapy of skin flaps with venous congestion in a rabbit model

作　　者：徐楠[1] 孙强[1] 王晨超[1] 金石峰[1] 王迪[1] 郭澍[1] 王玉新[1] 

机构地区：[1]中国医科大学附属第一医院整形外科,辽宁沈阳110001

出　　处：《中国美容整形外科杂志》2015年第2期68-71,共4页Chinese Journal of Aesthetic and Plastic Surgery

基　　金：基金项目：国家自然科学基金资助项目（51272286）;辽宁省自然科学基金资助项目（20102296）

摘　　要：目的 观察脂肪间充质干细胞移植对兔耳静脉淤血皮瓣的治疗效果.方法 分离兔脂肪间充质干细胞并采用流式细胞术鉴定CD90、CD29、CD31、CD34、CD45以及CD13的表达;建立兔耳静脉淤血皮瓣模型,治疗组给予脂肪间充质干细胞原位注射治疗,对照组给予细胞培养液,于术后7d切取治疗组及对照组的皮瓣组织,观察皮瓣成活情况并计算成活率;HE染色比较两组皮瓣的病理改变并计数微血管数目;免疫组化检测两组皮瓣组织中VEGF的表达情况.结果 所分离的脂肪间充质干细胞CD90、CD29、CD13表达阳性,而CD31、CD34以及CD45呈阴性表达;造模7d后,对照组仍可见明显的肿胀及淤血坏死且静脉内存在血栓,而治疗组坏死面积明显缩小,血栓程度减轻且可见新血管的形成,皮瓣成活率以及微血管密度均显著高于对照组（P＜0.05）;此外,治疗组VEGF的水平也高于对照组.结论 脂肪间充质干细胞移植能够促进皮瓣内VEGF的分泌,促进新血管的形成,从而改善静脉淤血,提高皮瓣的成活率.Objective To investigate the effect of adipose derived mesenchymal stem cells （ADSCs） on the therapy of skin flaps with venous congestion in a rabbit model. Methods Rabbit ADSCs were isolated and the expression of CD90, CD29, CD31, CD34, CD45 and CD13 was identified by flow cytometry. Rabbit venous congestion skin flaps model was established and ADSCs was the situ injected into the the experimental group, while the cell euhure fluid was injected into the control group. The flaps were cut and survival rate was measured at 7 days after surgery. Pathological changes of the flap tissues were observed by HE staining and the number of microvessels was counted. In addition, the expression of VEGF was detected by immunohistochemis- try. Results The expression of CD90, CD29 and CD13 was positive and CD31, CD34 and CIM5 was negative in the isolated ADSCs. Obvious swelling, bruising necrosis and venous thrombosis was observed in the flaps of the control group, but necrotic area, thrombus was significantly reduced in the ADSCs group. The flap survival rate and microvessel density of the ADSCs group was increased significantly compared to the control group （ P〈 0. 05 ）. Otherwise, the expression of VEGF in ADSCs was also obviously higher than control group. Conclusion ADSCs transplantation can promote the secretion of VEGF, ameliorate venous congestion and improve survival of the venous congestion skin flaps.

关 键 词：静脉淤血皮瓣 脂肪间充质干细胞 皮瓣成活 

分 类 号：R318[医药卫生—生物医学工程]