鼠胚实验在新建体外受精胚胎培养室质量控制中的作用  

The Role of Mouse Embryo Assay in the Quality Control of a Newly Constructed Laboratory for IVF Embryo Culture

作　　者：王辉田[1] 李涛[1] 黎伟豪[1] 欧建平[1] 

机构地区：[1]中山大学附属第三医院生殖医学中心,广东广州510630

出　　处：《实用妇产科杂志》2015年第2期115-119,共5页Journal of Practical Obstetrics and Gynecology

摘　　要：目的:检测新建体外受精实验室培养体系的可靠与否,是否符合人类胚胎体外培养的要求。方法:对昆明白小鼠进行超促排卵,手术获取昆明白小鼠雌雄配子进行体外受精和体内受精的胚胎;并进行体外培养观察两组鼠胚的受精率、2-细胞率、囊胚率及采用密度梯度离心法和上游法两种精液处理方法对小鼠体外受精胚胎发育情况的影响。结果:进行了30个周期的体内受精体外培养实验,共获卵1136枚;进行了35个周期的体外受精实验,共获卵1486枚。体内受精组获得的受精率(93.13±1.46)%和2-细胞率(94.52±1.67)%高于体外受精组的受精率(84.39±2.87)%和2-细胞率(87.80±3.21)%,两组间差异有统计学意义(P<0.05)。密度梯度离心法处理精液后,其受精率(90.32±3.14)%和2-细胞率(88.54±2.28)%高于上游法的受精率(76.46±2.35)%和2-细胞率(82.67±2.62)%,两者差异有统计学意义(P<0.05)。结论:小鼠卵母细胞体内受精和体外受精后均有高的成胚率,体内受精后进行体外培养优于体外受精后培养,采用密度梯度离心处理精液实施体外受精优于上游法,鼠胚实验可对新建胚胎培养室的培养体系进行评估。Objective： To test the reliability of the embryo culture system of a newly constructed laboratory for IVF embryo culture and to testify whether it conforms to the requirement of in vitro cultivation of human embryo or not. Methods： Superovulation treatments with PMSG and HCG on Kunming mouse were conducted. Then male and female gametes as well as pronuclear embryo were obtained from surgical approaches for in vivo and in vitro fertilization,respectively. The two groups of mouse embryos were cultured in vitro to observe the fertilization rate,2-cell rate and blastocyst rate. The effect of density gradient centrifugation and upstream two methods of semen treated on mouse embryonic development of in vitro fertilization. Results： Totally 1136 oocytes in 30-cycles of in vivo fertilization and totally 1486 oocytes in 35-cycles of in vitro fertilization were obtained. In vivo the fertilization rate was（ 93. 13±1. 46） % and 2-cell rate was（ 94. 52±1. 67） % were higher than that in vitro the fertilization rate was（ 84. 39±2. 87） % and 2-cell rate（ 87. 80 ±3. 21） %,the differences between the two groups were statistically significant（ P〈 0. 05）. There were statistically significant difference in fertilization rate（ 90. 32 ± 3. 14 vs 76. 46 ±2. 35） % and 2-cell rate（ 88. 54±2. 28 vs 82. 67±2. 62） % between destiny gradient centrifugation group and upstream method group（ P〈0. 05）. Conclusions： The embryo development of in vivo and vitro fertilization mouse oocytes and two methods of semen processing have reached the requirements of indoor quality control,the results are better in vitro culture of vivo fertilization and density gradient centrifugation processing semen. So mouse embryo assay could used to evaluate the embryo culture system in the newly constructed assisted reproductive laboratory.

关 键 词：鼠胚实验 体外受精 质量控制 胚胎培养 

分 类 号：R332[医药卫生—人体生理学]