LC-MS/MS法同时测定人血浆中4种肾上腺素β受体阻滞药的浓度  

Simultaneous determination of four β-adrenergic blockers drugs in human plasma by LC-MS / MS

作　　者：李力[1] 陈玲玲[1] 李贺[1] 刘炜[1] 

机构地区：[1]浙江医院药剂科浙江省老年医学重点实验室,杭州310013

出　　处：《中国临床药学杂志》2015年第1期13-17,共5页Chinese Journal of Clinical Pharmacy

基　　金：国家科技重大专项项目-老年病新药临床技术平台(编号2013ZX09303005)

摘　　要：目的建立LC—MS／MS法同时测定人血浆中阿罗洛尔、阿替洛尔、比索洛尔和美托洛尔的浓度。方法人血浆样晶用乙酸乙酯萃取后，选用ZORBAXSBC18（100mm×2．1mm，3．5μm）色谱柱，以甲醇-0．1％甲酸为流动相梯度洗脱，采用电喷雾离子化源、正离子方式和多反应监测（MRM）扫描方式进行监测。结果血浆中阿罗洛尔、阿替洛尔、比索洛尔和美托洛尔的线性范围均为1．0～500μg·L^-1（r〉0．99），定量下限均为1．0μg·L^-1；平均回收率94．4％～107．9％；日内、日间RSD均〈15％；提取回收率在78．8％。89．4％。结论该方法快速、灵敏、准确，专属性强，重复性好，适用于人血浆中同时测定阿罗洛尔、阿替洛尔、比索洛尔和美托洛尔浓度。AIM To develop an LC-MS/MS method for simultaneous determination of arotinolol, atenolol, biso- prolol and metoprolol concentrations in human plasma. METHODS The plasma samples were processed with ethyl ac- etate extraction. The separation was achieved on ZORBAX SB C18 column （100 mm× 2.1 mm, 3.5μm） and eluted with linear gradient using methanol and 0.1% formic acid. Detection of the analytes were achieved using positive ion electro- spray ionization （ESI） in the multiple reaction monitoring （MRM） mode. RESULTS The linear calibration curves of arotinolol, atenolol, bisoprolol and metoprolol were obtained in the concentration range of I. 0 - 500 μg· L- 1 （ r 〉 0.99）, with the lower limit of quantitation （LLOQ） of 1.0μg· L-1. The average recovery was between 94.4 % - 107.9 %. Intra- day and inter-day relative standard deviations were both below 15 %. The recoveries of low, middle and high concentra- tions were 78.8 % - 89.4 %, respectively. CONCLUSION The established method is rapid, sensitive, accurate, spe- cific and reliable, and suitable for simultaneous determination of arotinolol, atenolol, bisoprolol and metoprolol in human plasma.

关 键 词：阿罗洛尔 阿替洛尔 比索洛尔 美托洛尔 液质联用法 血药浓度 

分 类 号：R969[医药卫生—药理学]