七十味珍珠丸对大鼠脑缺血再灌注损伤的抗凋亡作用  

Anti-apoptosis effect of Rannasangpei on brain ischemia and reperfusion injury in rats

作　　者：朱敏侠[1] 刘晓丽[1] 戎浩[1] 李杨[1] 李捷[1] 贺学[1] 盛业萌[1] 

机构地区：[1]西藏民族学院医学院,陕西咸阳712082

出　　处：《天津医药》2015年第2期150-153,共4页Tianjin Medical Journal

基　　金：西藏自治区科技厅2010年第二批重点科研项目(藏科发[2010]154号);西藏民族学院院级科研项目(10my Y07)

摘　　要：目的观察七十味珍珠丸(RNSP)对大鼠脑缺血再灌注损伤的保护作用及抗凋亡机制。方法采用大脑中动脉阻塞模型,实验分组:假手术组、模型组、溶媒对照组和RNSP组。神经功能缺失评分观察大鼠神经缺失体征,红四氮唑染色测量脑组织梗死面积,及Western blot检测海马组织Bcl-2/Bax比值和caspase-3的蛋白表达。结果与假手术组比较,模型组大鼠神经缺失体征明显,脑梗死面积明显增加,Bcl-2/Bax比值减小,caspase-3蛋白表达明显升高。而与模型组比较,RNSP组大鼠神经缺失体征明显改善,脑梗死面积减少,Bcl-2/Bax比值增大,caspase-3蛋白表达明显降低。结论 RNSP对大鼠脑缺血再灌注损伤具有保护作用,与其上调Bcl-2/Bax比值,减少caspase-3蛋白表达的抗凋亡作用有关。Objective To observe the protective and anti-apoptosis effects of Rannasangpei（RNSP） on brain ischemiaand reperfusion injury in rats. Methods Middle cerebral artery occlusion（MCAO） model was established and the groupswere divided as sham group, MCAO group, vehicle ＋ MCAO group and RNSP ＋ MCAO group. Neuronal deficient signs,brain infarct area, the ratio of Bcl-2/Bax and the expression of caspase-3 were evaluated by neuronal deficient score, TTC（2,3,5-Triphenyltetrazolium chloride） staining and Western blot respectively. Results Compared with those parameters insham group, the neuronal deficient signs, infarct area and caspase-3 expression increased evidently while the ratio of Bcl-2/Bax decreased markedly in MCAO group. But in RNSP ＋ MCAO group, the neuronal deficient signs, infarct area and cas-pase-3 expression decreased greatly while the ratio of Bcl-2/Bax increased markedly compared with those parameters inMCAO group. Conclusion RNSP may have protective effects on brain ischemia and reperfusion, which is related to its an-ti-apoptosis role indicated by upregulation of Bcl-2/Bax ratios and downregulation of caspase-3.

关 键 词：脑缺血 再灌注损伤 细胞凋亡 大鼠 Sprague-Dawley 七十味珍珠丸 

分 类 号：R285.5[医药卫生—中药学]