绵羊感染伪狂犬病的病理学观察与病毒分离鉴定  

Pathological Observation of Sheep Infected Pseudorabies Virus,and Isolation and Identification of the Virus

作　　者：齐新永[1] 刘建[1] 周锦萍[1] 鞠厚斌[1] 葛菲菲[1] 张维谊[1] 徐峰[1] 沈莉萍[1] 宁昆[1] 王建[1] 刘佩红[1] 

机构地区：[1]上海市动物疫病预防控制中心,上海201103

出　　处：《中国畜牧兽医》2015年第2期365-369,共5页China Animal Husbandry & Veterinary Medicine

基　　金：沪农科推字(2012)第3-2号

摘　　要：为了确诊1例疑似绵羊伪狂犬病病例,本试验以上海郊区某羊场发病绵羊的病料为研究对象进行了病理学观察、病毒分离和鉴定。病理组织学变化显示,发病羊大脑组织神经元发生广泛性变性、坏死并伴有嗜神经现象,神经元周围出现胶质细胞增生。病料接种BHK-21细胞,细胞出现病变,间接免疫荧光试验和荧光定量PCR检测结果证实所分离的病毒为伪狂犬病病毒。流行病学调查结果表明,绵羊伪狂犬病可能是由猪伪狂犬病病毒感染引起的。通过免疫接种猪伪狂犬病弱毒疫苗,绵羊伪狂犬病疫情得到及时控制。To diagnose a suspected sheep pseudorabies case, the tissue samples of sheep with clini- cal symptom of sheep pseudorabies from a farm in Shanghai suburb were collected for pathological observation,isolation and identification of the virus. The histopathological observation of sheep showed extensive neuronal degeneration and necrosis occurred in the brain tissue accompanied with neurotropic phenomenon and gliosis. BHK-21 cells inoculated by tissue samples showed lesions. Pseudorabies virus （PRV） in BHK-21 cells was confirmed by specific fluorescence of indirect immunofluorescence assay and fluorescence PCR. Epidemiological investigations showed sheep pseudorabies was possibly caused by swine pseudorabies virus. Sheep pseudorabies was controlled in time by inoculating swine pseudorabies attenuated vaccine.

关 键 词：绵羊伪狂犬病 病理组织学 细胞培养 荧光定量PCR 

分 类 号：S855.3[农业科学—临床兽医学]