基于微悬臂梁生物传感器检测三磷酸腺苷  

Microcantilever Biosensor for Detection of Adenosine Triphosphate

作　　者：李辉彦 白效静[2] 王风红[1] 李静[1] 唐纪琳[2] 

机构地区：[1]吉林大学公共卫生学院卫生检验学教研室,长春130021 [2]中国科学院长春应用化学研究所电分析国家重点实验室,长春130022

出　　处：《应用化学》2015年第3期362-366,共5页Chinese Journal of Applied Chemistry

摘　　要：基于适配子构建了无标记检测三磷酸腺苷（ATP）的微悬臂梁生物传感器。将ATP适配子修饰在微悬臂梁阵列中的传感悬臂镀金面上,用来识别ATP,而参比悬臂修饰巯基己醇（MCH）防止非特异性吸附。ATP与其适配子发生特异性相互作用,使悬臂的上下两个表面产生应力差,导致传感悬臂产生偏转,扣除参比悬臂偏转后其偏转值与ATP的浓度在0.5~5 mmol/L范围内有良好的线性关系,相关系数为0.998,最低检出限为0.06 mmol/L。该微悬臂梁生物传感器响应快速、操作简单,并且对ATP具有良好的特异性。An aptamer-based microcantilever biosensor for label-free detection of adenosine triphosphate （ATP） was developed. The sensor cantilevers in the microcantilever array were functionalized with ATP aptamer on the gold side to recognize ATP, while the reference cantilevers were modified by 6-mercapto-1- hexanol（MCH） to prevent non-specific absorption. The interaction between ATP and aptamer on the sensor cantilerers gold surface induced surface stress change and reference cantilevers. The differential deflection s, resulting in a differential deflection between the sensor has a good 1}near relationship with the ATP concentration ranging from 0. 5- 5 mmol/L with a correlation coefficient of 0. 998. The detection limit is about 0. 06 mmol/L. The aptamer-based microcantilever sensor shows rapid response, simple procedures and high specificity for ATP.

关 键 词：三磷酸腺苷 适配子 微悬臂梁生物传感器 表面应力 

分 类 号：O655.9[理学—分析化学]