豚鼠感染鼠疫菌脏器的病理改变  

Pathological changes in solid viscera of Guinea pigs infected with Yersinia pestis

作　　者：赵忠智[1] 于守鸿[1] 张爱萍[1] 党占翠[2] 王梅[1] 郑谊[1] 熊浩明[1] 于志敏[3] 赵海龙[2] 王祖郧[1] 

机构地区：[1]青海省地方病预防控制所鼠疫预防控制科,西宁811602 [2]青海大学医学院 [3]青海大学附属医院

出　　处：《中国媒介生物学及控制杂志》2015年第1期84-85,共2页Chinese Journal of Vector Biology and Control

摘　　要：目的探讨豚鼠感染鼠疫菌主要实质性脏器的病理改变。方法强毒鼠疫菌25 cfu/ml感染成体实验豚鼠鼠蹊部皮下。病死豚鼠立即取材,未病死豚鼠14 d分别处死并取材。取肝、脾、肺、肾及心等实质性脏器分离培养鼠疫菌,并制作病理切片观察病理改变。结果感染鼠疫菌的实验豚鼠肝、脾、肺、肾及心脏均分离出鼠疫菌;其组织巨检均有明显病理变化,镜检除心脏外均有明显的病理变化,呈炎性细胞增多,充血、出血、部分坏死等急性炎症变化。结论强毒鼠疫菌引起实验豚鼠急性炎症反应,豚鼠肝、脾、肺、肾、心等实质性脏器的病理变化显著。Objective To investigate pathological changes in the major solid viscera of guinea pigs infected with the plague bacillus Yersinia pestis.MethodsAdult guinea pigs were infected with a virulent Y. pestis strain（25 cfu/ml） through the groin subcutaneously. After infection, tissue specimens were immediately taken from dead animals, and the surviving ones were executed 14 d later. Specimens of solid viscera, including the liver, spleen, lung, kidney, and heart, were used for isolation of Y. pestis, and section specimens were prepared to observe associated pathological changes.Results Yersinia pestis was isolated from the liver, spleen, lung, kidney, and heart of the experimental animals after infection. Tissue autopsy revealed significant pathological changes in all solid viscera, and microscopic examination showed obvious pathological changes in the major organs other than the heart. There were acute inflammatory changes such as inflammatory cell increase, hyperemia,hemorrhage, and partial necrosis.ConclusionVirulent Y. pestis can cause acute inflammation in guinea pigs and result in significant pathological changes in solid viscera of the animals, including the liver, spleen, lung, kidney, and heart.

关 键 词：鼠疫耶尔森菌 组织病理学 

分 类 号：R254.8[医药卫生—中医内科学]