维生素C反相微乳液的构建及红外光谱研究  

Construction and FT-IR Investigation of Vitamin C Reverse Microemulsions

作　　者：孟照永 王如梅[1] 赵新[1] 韩正[1] 王仲妮[1] 

机构地区：[1]山东师范大学化学化工与材料科学学院,山东济南250014

出　　处：《食品与药品》2015年第1期1-4,共4页Food and Drug

基　　金：国家自然科学基金(31271933);国家级大学生创新训练计划项目(201310445068)

摘　　要：目的分别构筑以乙酸异戊酯、肉豆蔻酸异丙酯、葵花籽油和蓖麻油为连续相的包载维生素C(Vit C)微乳液,并研究其红外光谱(IR)性质。方法采取水稀释法构筑三元相图,研究非离子表面活性剂油烯基聚氧乙烯(10)醚(Brij97)水溶液在乙酸异戊酯、肉豆蔻酸异丙酯、葵花籽油、蓖麻油4种不同油相中的相行为;以Brij97、不同油相与Vit C形成反相微乳液,根据Vit C增溶前后IR图谱变化,并通过对羟基伸缩振动峰进行高斯曲线拟合,分析体系微环境的变化。结果 Brij 97分别在4个不同结构的油相体系中形成了反相微乳液。IR光谱分析表明,Vit C增溶后O-H伸缩振动峰在高频区增加了第4个高斯峰,在葵花籽油和蓖麻油体系中,4号新峰的位置随体系油含量的降低,向低频区位移;而在乙酸异戊酯和肉豆蔻酸异丙酯体系中,随体系油含量变化无规律波动。结论乙酸异戊酯和肉豆蔻酸异丙酯为连续相的微乳液形成效率高于葵花籽油和蓖麻油体系;IR分析表明Vit C被完全增溶于反相微乳液的亲水内核中;高斯分峰拟合表明Vit C的引入导致体系微环境增加了一种结合水。Objective To construct Vitamin C （Vit C） encapsulated microemulsions by employing isoamyl acetate, isopropyl myristate, sunflower oil or castor oil as the continuous phase and analyze their infrared spectral （IR） properties. Methods The ternary phase diagrams were constructed by water-dilution method to determine the phase behaviors of aqueous solution of nonionic surfactant polyoxyethylene （10） oleyl ether （Brij97） in isoamyl acetate, isopropyl myristate, sunflower oil and castor oil; the reverse emulsions were formed with Brij97, oil phase and Vit C, and the structural difference of the microemulsion systems with or without Vit C was investigated through FT-IR; the O-H stretching vibration peak and micro-environment of each microemulsion system were analyzed using Gaussian curve fitting method. Results Reverse microemulsions have been formed with Brij97 aqueous solution in four oils respectively. FT-IR measurements and analyses indicated after solubilization of Vit C, the fourth new Gaussian peak in high frequency zone is obtained for the O-H stretching vibration of the microemulsion, The new peak waved irregularly along with changes of oil concentration in the isoamyl acetate and isopropyl myristate systems, while moved to lower frequency region along with the decrease of oil content for the sunflower oil and castor oil systems. Conclusion It is more effective in forming reverse microemulsion with isoamyl acetate and isopropyl myristate as continuous phase than with sunflower oil or castor oil as continuous phase. In addition, FR-IR spectra showed that Vit C was completely wrapped into the hydrophilic kernel of reverse microemulsion, and the multipeak fitting results showed that the introduction ofVit C resulted in a new combined water in the microenvironment of the system.

关 键 词：维生素C 反相微乳液 红外光谱 

分 类 号：R943[医药卫生—药剂学]