绵羊骨骼肌特异表达miR-133前体在不同绵羊群体中的多态检测  

Detection and Structure Prediction of Polymorphism at Skeletal Muscle-specific Pri-miRNA-133 among Different Sheep Breeds

作　　者：宋广超[1,2] 张伟[1,2] 许瑞霞[1,2] 赵伟利[1,2] 刘守仁[1,2] 甘尚权[2] 

机构地区：[1]石河子大学动物科技学院,新疆石河子832003 [2]新疆农垦科学院畜牧兽医研究所,新疆石河子832000

出　　处：《家畜生态学报》2015年第1期18-23,共6页Journal of Domestic Animal Ecology

基　　金：国家自然科学基金地区基金(31160452;31060303);新疆农垦科学院引导计划(YYD2010-9)

摘　　要：试验旨在研究骨骼肌中特异表达miR-133前体在不同绵羊群体中的多态,分析其与绵羊骨骼肌发育表型的相关性。采用PCR-SSCP技术对miR-133前体序列在特克赛尔羊、中国美利奴细毛羊、阿勒泰羊和湖羊四种不同产肉性能的绵羊群体进行了多态性检测,并分析多态性位点对miR-133前体序列二级结构、二级结构稳定性及加工、成熟的影响。结果表明,miR-133前体序列下游194bp(g.54047535)处检测到A>G突变,并且该SNP与绵羊的产肉性能存在明显的相关性,在特克赛尔羊中GG是优势基因型,G等位基因频率高达0.86,是中国美利奴细毛羊群体该位点频率的4倍。二级结构的生物信息学预测结果表明,该SNP位点致使miR-133前体的二级结构发生明显改变,自由能值降低3.2kJ/mol。以上结果提示,绵羊miR-133前体序列的SNP(g.54047535)位点可能对miR-133前体的加工成熟具有重要影响,并最终影响绵羊的产肉性能。To study the polymorphism of skeleton muscle-specific pri-miR-133 among different sheep breeds and analyze its correlation with the muscle phenotype, pri-miR-133 polymorphism among four breeds with different meat production performance （Texel, Chinese Merifio Fine Wool, Altay and Hu Sheep）, and the effects of the pri-miR-133h polymorphism on its secondary structure, stability and the ca- pacity of mature miR-133 processing were detected and analyzed by PCR-SSCP. The result showed that there was a A/G transversion at 194 bp downstream of pre-miR-133b, and the SNP was clearly associated with its corresponding meat traits. In Texel flock, GG genotype was the preponderant genotype, and G al- lele frequency reached 0.86, the value of G allele frequency in Texel was four times the frequency of G al- lele in Chinese Merino flock. The Secondary structure further bio informatics analysis showed that the mu- tation significantly changed the secondary structure of pre-miR-133, and the free energy value was reduced by 3.2 KJ/mol. The above indicated that the SNP in pri-miR-133 may have a significant impact on the pro- cessing of mature miR-133, and affect the performance of sheep meat production.

关 键 词：pri—miRNA pre—miRNA miR-133 多态性 骨骼肌 绵羊 

分 类 号：S811.6[农业科学—畜牧学]