载有槲皮素的PEG-PE胶束对乳腺癌细胞耐药性的逆转效应  

Quercetin-loaded PEG-PE micelles reverse drug resistance of MCF-7 ADRr human breast cancer cells

作　　者：吴金花[1] 段金虹[1] 许海燕[2] 杨先达[1] 

机构地区：[1]中国医学科学院北京协和医学院基础医学研究所病理学与病理生理学系,北京100005 [2]中国医学科学院北京协和医学院基础医学研究所生物医学工程学系,北京100005

出　　处：《基础医学与临床》2015年第2期174-177,共4页Basic and Clinical Medicine

基　　金：科技部重大科学计划(2011CB933504)

摘　　要：目的探索载有槲皮素的PEG-PE聚合物胶束(M-Q)能否在体外逆转耐阿霉素人乳腺癌细胞(MCF-7 ADRr)的耐药性。方法将PEG-PE聚合物与槲皮素混合制备脂膜,加0.9%氯化钠注射液形成胶束,用动态光散射粒径仪表征其尺寸分布,并将其和阿霉素与MCF-7 ADRr细胞共孵育72 h,用MTS方法检测对肿瘤细胞的杀伤率。结果PEG-PE聚合物胶束能有效运载槲皮素,其包封率高达74%以上,平均粒径为11.11 nm左右,与游离槲皮素相比,M-Q能在体外更大程度地逆转MCF-7 ADRr细胞对于阿霉素的耐药性(P<0.05),且PEG-PE胶束自身对靶细胞无明显杀伤作用。结论载有槲皮素的PEG-PE胶束在逆转肿瘤耐药性方面具有应用潜能。Objective To explore whether quercetin-loaded PEG-PE micelles（ M-Q） can synergize the growth-inhibitory activity of adriamycin prepared（ ADR） by reversing the drug resistance of MCF-7 ADRr breast cancer cells in vitro. Methods M-Q was prepared by adding saline to lipid film containing quercetin and PEG-PE. The size of M-Q was characterized by dynamic light scattering（ DLS）. The inhibition of MCF-7 ADRr cells was evaluated by MTS assay after incubation with M-Q and ADR. Results The incorporation efficiency of quercetin by the micelles was above 74%. The average size of M-Q was 11. 11 nm. Compared with the quercetin dissolved in ethanol,M-Q more effectively reversed the drug resistance of MCF-7 ADRr cells in vitro. Conclusions PEG-PE micelles may potentially deliver quercetin to cancer cells for reversal of drug resistance.

关 键 词：PEG-PE 胶束 槲皮素 MCF-7 ADRr细胞 

分 类 号：R285[医药卫生—中药学]