宝坻大蒜再生体系优化及性能评估  

Optimization and Performance Evaluation of the Regeneration System of the Baodi Garlic

作　　者：范宝莉[1] 王珍[1] 任春雪[1] 顾增惠[1] 刘晓颖[1] 王振英[1] 

机构地区：[1]天津师范大学生命科学学院,天津300387

出　　处：《生物技术通报》2015年第2期97-102,共6页Biotechnology Bulletin

基　　金：天津市农委重点项目(5KN13001);天津师范大学应用开发研究基金项目;天津市科委面上项目(14JCYBJC29900)

摘　　要：通过对分化培养基和生根培养基进行优化,完善了宝坻大蒜再生体系,确定最佳分化培养基为MS+6-BA 5.0 g/L+NAA 1.0 g/L,最佳生根培养基为无激素的MS培养基。对再生植株进行细胞学鉴定及田间评估,未发现染色体变异,再生植株田间特性有利于提高光合效率。The regeneration system of Baodi garlic was improved by optimizing the differentiation medium and rooting medium. The optimal differentiation medium was MS＋6-BA 5.0 g/L＋NAA 1.0 g/L and the best medium for rooting was MS medium without hormones. No chromosomal aberration was observed according to the results of the cytological identification and field assessment of the plant regeneration. The field features indicated the improvement of the photosynthetic efficiency of the plant regeneration.

关 键 词：宝坻大蒜 再生植株 叶夹角 鳞茎 

分 类 号：S633.4[农业科学—蔬菜学]