海州香薷Actin基因片段克隆及表达分析  

Cloning and Expression Analysis of Actin Gene Gragment from Elsholtzia haichowensis

作　　者：蔡深文[1] 熊治廷[2] 刘晨[2] 徐仲瑞[2] 邓松强[2] 

机构地区：[1]遵义师范学院资源与环境学院,遵义563002 [2]武汉大学资源与环境科学学院,武汉430079

出　　处：《生物技术通报》2015年第2期111-115,共5页Biotechnology Bulletin

基　　金：国家自然科学基金项目(30870365;31270432)

摘　　要：通过克隆海州香薷Actin基因片段并分析其组织表达,为研究海州香薷重金属抗性相关基因的表达调控奠定基础。根据Gen Bank中其他植物Actin基因保守序列设计兼并引物,以海州香薷根总RNA为模板,利用RT-PCR技术分离得到Actin基因片段。序列分析结果表明,海州香薷Actin基因片段长576 bp,编码192个氨基酸,与其他植物同源基因的氨基酸序列相似性为84%-97%,所克隆的序列为Actin基因的同源片段,将其命名为Eh ACT,在Gen Bank中提交序列,获得登录号AGT37260。半定量RT-PCR分析结果表明,Eh ACT在海州香薷的根、茎和叶中表达相对稳定,初步表明其可作为研究海州香薷基因表达的内参基因。Cloning and expression analysis of Actin gene fragment from Elsholtzia haichowensis would provide foundation for the study of gene expression and regulation of heavy metal resistance related genes. Degenerate primers were designed based on the conserved sequences of the Actin genes from other plants. Total RNA was extracted from the root of E. haichowensis. An Actin gene fragment was separated by reverse transcription polymerase chain reaction（RT-PCR）. The sequence analysis results revealed that Actin gene fragment from E. haichowensi contains 576 bp, encoding a protein of 192 amino acids. Homology comparison with other plants Actin gene sequences in the GenBank showed that it shared 84%-97%amino acid sequence homology with other plants. The cloned sequence was Actin gene fragment. It was named as EhACT and was registered into GenBank（accession number：AGT37260）. Semi-quantitative PCR assays indicated that the expression of EhACT in root, stem and leaf of E. haichowensis was relatively stable, suggesting that EhACT can be used as the reference to analyze the gene expression in E. haichowensis.

关 键 词：海州香薷 ACTIN基因 克隆 表达 

分 类 号：Q943.2[生物学—植物学]