斯达油脂酵母中自噬与油脂积累的关联性分析  

An Investigation of Cellular Autophagy and Oil Accumulation in the Oleaginous Yeast Lipomyces starkeyi

作　　者：韩冰[1] 杨琴[1] 崔清华[1] 

机构地区：[1]云南大学生命科学学院,昆明650091

出　　处：《生物技术通报》2015年第2期208-216,共9页Biotechnology Bulletin

基　　金：云南省创新团队基金项目(2011C1123);国家自然科学基金项目(31160237)

摘　　要：旨在证明斯达油脂酵母中自噬参与油脂积累过程。在不同油脂积累水平下,检测相关自噬基因的表达,观察自噬与油脂积累是否有潜在相关性;用自噬促进剂促进自噬后,观察酵母油脂积累水平是否有差异。结果表明,与油脂低积累水平相比,在油脂高积累条件下,斯达油脂酵母自噬水平较低;用自噬促进剂处理后,酵母油脂积累降低。在斯达油脂酵母中,自噬与油脂积累成负相关性,自噬水平的提高会使酵母中油脂积累降低。It was to prove autophagy is involved in the accumulation of lipid in Lipomyces starkeyi. Detecting the expression of autophagy-related gene in different lipid accumulation level to determine whether autophagy is potentially involved in lipid accumulation; promoting autophagy by autophagy accelerator to observe whether there are differences in yeast lipid accumulation level. Results showed that the level of autophagy is low in high lipid accumulation condition compare with low condition. After treatment with autophagy accelerator, the lipid accumulation in yeast is reducing. In Lipomyces starkeyi, autophagy is negatively related to lipid accumulation, the improving of autophagy level will reduce the lipid accumulation in yeast.

关 键 词：自噬 斯达油脂酵母 油脂积累 泛素样共轭体系 

分 类 号：Q93[生物学—微生物学]