血液杆菌Haematobacter sp.细胞脂肪酸测定的影响因素  

The Influence Factors of Determination of Haematobacter sp. Cellular Fatty Acids

作　　者：何蔚荭[1] 安明理[1] 陈国参[1] 贾彬[1,2] 吴晓磊[3] 王亚南[1] 

机构地区：[1]河南省科学院生物研究所有限责任公司,河南省微生物工程重点实验室,郑州450008 [2]深圳大学生命科学学院,深圳518060 [3]北京大学工程学院,北京100871

出　　处：《生物技术通报》2015年第2期217-221,共5页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31100582);河南省基础与前沿技术研究计划项目(102300413217)

摘　　要：应用美国MIDI公司Sherlock MIS系统检测一株归属于血液杆菌属的新菌种Haematobacter sp.HNMC11807的细胞脂肪酸组成,探讨不同培养方法和条件对其细胞脂肪酸组成的影响。采用不同培养条件培养微生物细胞,即不同的培养基组成、固液类型和培养时间;分析比较细胞脂肪酸检测结果存在的差异。同时对比分析仪器专用的两种检测体系,即常规标准和快速标准体系,对样品脂肪酸测定结果的影响。结果发现,细胞脂肪酸组成与其培养条件密切相关,且差异显著。因此,在应用该系统做微生物分类鉴定时,必需严格遵守特定的、统一的培养条件平行进行。Cellular fatty acid composition of strain HNMC11807, a novel species belong to Haematobacter sp., were detected by used Sherlock MIS system of MIDI company USA. The influence by different culture methods and cells growth conditions on cells fatty acid composition were explored in this article. Based on the bacterial cells cultured in varied medium such as in different medium components and in solid or liquid medium, the cellular fatty acid composition were respectively analysed and compared. On the same time, two standard systems in this instrument, conventional and fast, were used to run samples, and the detect results of the cellular fatty acid composition were compared respectively. As the results, the cells fatty acid composition was closely related with culture condition and a significant differences. The cellular fatty acid composition must be carried out in specified and unified culture conditions when this system was used in microbial classification.

关 键 词：细胞脂肪酸检测 培养基条件 脂肪酸标准品 血液杆菌 

分 类 号：Q936[生物学—微生物学]