丝素蛋白对基于聚左旋乳酸电纺织丝纳米支架的生物相容性的影响  

In vivo biocompatibility characteristic of PLLA-based electronspun nanofibrous scaffolds blended with SF

作　　者：程自申[1] 杨成伟[1] 刘莉[2] 杨洋[1] 邓国英[1] 叶晓健[1] 

机构地区：[1]第二军医大学长征医院骨科,上海200003 [2]河北省保定市第一中心医院妇产科

出　　处：《山西医药杂志》2015年第3期243-246,I0001,共5页Shanxi Medical Journal

基　　金：国家自然科学基金(81472071)

摘　　要：目的系统地研究丝素蛋白(SF)对基于聚左旋乳酸(PLLA)的纳米纤维支架体内生物相容性的影响。方法通过静电纺丝的技术分别制备聚左旋乳酸-共轭-ε-己内酯[P(LLA-CL),质量比=1∶1]及混有含25%SF的SF/P(LLA-CL)纳米纤维支架。2种支架分别分别植入45只6个月龄大鼠皮下6个月,评估生物相容性。结果病理切片显示,SF能显著改善组织反应,表现为1、3个月时SF/P(LLA-CL)支架较P(LLA-CL)支架有较少的巨噬细胞侵入及3个月时在SF/P(LLA-CL)支架周围较少的异物巨细胞的形成,同样还发现肿瘤坏死因子-α、转化生长因子-β或白细胞介素(IL)-1β和IL-10在不同的植入时间有较低的表达水平。结论 SF加入P(LLA-CL)支架后在皮下植入可减轻炎症反应、改善生物相容性,提示SF可作为材料设计具有前景的物质。Objective To systematically investigate the effect of silk fibroin（SF）on biocompatibility of poly-L-lactic-acid（PLLA）-based nanofibrous scaffolds in vivo.Methods The scaffolds of poly [L-lactic acid-co-ε-caprolactone）P（LLA-CL）,w/w =1∶1]blended with 25% of SF [SF/P（LLA-CL）and P（LLA-CL）]were prepared by electrospinning.The P（LLA-CL）and SF/P（LLA-CL）scaffolds were subcutaneously implanted in 45 6-month-old rats for up to 6months to evaluate their biocompatibility characteristics.Results Pathological sections showed that SF incorporation obviously improved the tissue response after subcutaneous implantation where we observed less macrophage infiltration at 1,3-month and less foreign body giant cell（FBGC）formation surrounding the scaffolds at 3-month around SF/P（LLA-CL）scaffolds compared with P（LLA-CL）scaffolds.Some similar differences were also observed for the relative expression levels for tumor necrosis factor（TNF）-α,transforming growth factor（TGF）-β,or interleukin（IL）-1βand IL-10 in SF/P（LLA-CL）scaffold groups at different implantation time.Conclusion The results of the study indicate that SF incorporation can reduce inflammation,improve biocompatibility in P（LLA-CL）scaffolds,suggesting that SF may be a highly promising scaffold material when the scaffold was designed.

关 键 词：丝素蛋白 巨噬细胞 巨细胞 异物 生物相容性材料 

分 类 号：R318.08[医药卫生—生物医学工程]