运用微型正电子发射扫描技术观察电针对SAMP8老年痴呆小鼠大脑海马区葡萄糖代谢的影响  

Micro-PET Observation of the Effect of Electroacupuncture on Cerebral Hippocampus Glucose Metabolism in SAMP8 Mice with Senile Dementia

作　　者：姜婧[1] 高凯[2] 周源[1] 韩向博 朴赞勋 李志刚[1] 

机构地区：[1]北京中医药大学针灸推拿学院,北京100029 [2]中国医学科学院医学实验动物研究所,北京100021

出　　处：《上海针灸杂志》2015年第2期176-179,共4页Shanghai Journal of Acupuncture and Moxibustion

基　　金：国家自然科学基金项目(81273825)

摘　　要：目的利用18氟-脱氧葡萄糖micro-PET（18F-FDG micro-PET）影像学技术观察正常组小鼠,模型组和电针组老年痴呆模型SAMP8小鼠（快速老化小鼠）大脑海马区的葡萄糖代谢情况。方法随机挑选6月龄正常组SAMR1小鼠4只,痴呆模型组和电针组SAMP8小鼠各4只,通过2%异氟烷吸入麻醉后,从尾静脉弹丸式注射放射性示踪剂18F-FDG,每例约14.8-16.5MBq,摄取1 h后进行10 min micro-PET图像采集。计算并比较各组小鼠每克脑的海马组织18F-FDG的摄取率。结果电针组小鼠每克脑组织18F-FDG的摄取率高于正常组和模型组。结论电针能明显提高SAMP8小鼠脑18F-FDG的摄取及每克脑组织的摄取率,并可能通过影响脑葡萄糖代谢而发挥神经保护作用。Objective To observe cerebral hippocampus glucose metabolism using 18F-FDG micro-PET as an imaging technique in a normal group of mice, and model and electroacupuncture groups of SAMP8 mice with senile dementia （rapid aging mice）. Method Four 6-month-old SAMR1 mice were picked at random out of the normal group and every four 6-month-old SAMP8 mice, out of the model and electroacupuncture groups respectively. Each of them was anesthetized by inhalation of 2% isoflurane and then given a bolus injection of radioactive tracer 18F-FDG 14.8-16.5 MBq through the tail vein. After one hour of the uptake, a PET scan was performed for 10 min. Hippocampus 18F-FDG uptake rate per gram of brain was calculated in every group of mice and the uptake rates were compared between the groups.Result The 18F-FDG uptake rate per gram of brain tissue was higher in the electroacupuncture group of mice than in the normal and model groups.Conclusion Electroacupuncture can markedly increase cerebral 18F-FDG uptake and the uptake rate per gram of brain tissue in SAMP8 mice. It may play a neuroprotective role through its influence on cerebral glucose metabolism.

关 键 词：18氟-脱氧葡萄糖 海马葡萄糖代谢 电针 SAMP8小鼠 阿尔茨海默病 

分 类 号：R2-03[医药卫生—中医学]