葡萄糖氧化酶在水溶性CdTe量子点上的直接电化学研究  

Direct Electrochemistry of Glucose Oxidase at Water-soluble Cd Te Quantum Dots

作　　者：余祎[1] 喻玖宏[1] 

机构地区：[1]武汉轻工大学化学与环境工程学院,湖北武汉430023

出　　处：《分析测试学报》2015年第2期194-199,共6页Journal of Instrumental Analysis

基　　金：生命分析化学教育部重点实验室开放基金(KLACLS1005)

摘　　要：利用合成的Cd Te量子点（QDs）作修饰材料,将葡萄糖氧化酶（GOD）固定在水溶性Cd Te量子点表面,制备了葡萄糖氧化酶Cd Te量子点修饰碳糊电极（GOD/Cd Te/CPE）,实现了GOD在电极表面的直接电化学。Cd Te QDs能有效地加速葡萄糖氧化酶（GOD）与电极表面的直接电子转移,电子传递效率比无QDs Cd Te存在时提高约8倍;电子转移速率常数（K）为0.14 s^-1,传递系数（α）为0.60,GOD在GOD/Cd Te/CPE表面的平均覆盖量（Γ）为7.9×10^-8mol/cm^2。GOD/Cd Te/CPE电极作为第三代葡萄糖电化学生物传感器,成功应用于葡萄糖浓度的检测,其线性范围为0.050-0.32 mmol/L,检出限为0.020 mmol/L。GOD/Cd Te/CPE的制备方法简单,稳定性强,具有优良的选择性和重现性,且响应速度快。In this paper, a glucose oxidase Cd Te quantum dots modified carbon paste electrode（ GOD/Cd Te /CPE） was prepared by the synthesis of Cd Te quantum dots（ QDs） to fabricate modified material and fixed the glucose oxidase（ GOD） at the surface of the water-soluble Cd Te quantum dots,and achieved the direct electrochemistry of GOD at the electrode surface. QDs Cd Te can effectively accelerate the direct electron transfer of GOD with the electrode surface,and the electron transfer efficiency was about 8 times more than that in the absence of QDs Cd Te. The electron transfer rate constant K was 0. 14 s^- 1 and transfer coefficient was 0. 60. The average amount of coverage（ Γ） of GOD on the surface of GOD / Cd Te / CPE was 7. 9 × 10^- 8mol / cm^2. As a third generation glucose biosensor,GOD / Cd Te / CPE was successfully applied in the detection of glucose concentration. The calibration curve was linear in the range of 0. 050- 0. 32 mmol / L and the detection limit was 0. 020 mmol / L.GOD / Cd Te / CPE had the advantages of simple preparation,strong stability,excellent selectivity and reproducibility,and fast response.

关 键 词：Cd Te量子点 葡萄糖氧化酶 电化学生物传感器 循环伏安 直接电化学 

分 类 号：O657.72[理学—分析化学] F767.4[理学—化学]