玉米醇溶蛋白/壳聚糖复合纳米微球的制备及性能研究  

Preparation and controllable release behaviors of zein / chitosan composite nanoparticles

作　　者：刘洁[1] 裴菲[1] 何玉凤[1] 徐知丽 李涛[1] 王荣民[1] 

机构地区：[1]生态环境相关高分子材料教育部重点实验室 甘肃省高分子材料重点实验室 西北师范大学化学化工学院,甘肃兰州730070

出　　处：《化学研究与应用》2015年第2期184-188,共5页Chemical Research and Application

基　　金：国家自然科学基金(21263024,21364012)

摘　　要：以生物相容性的玉米醇溶蛋白(Zein)和壳聚糖(Chitosan)为原料,利用溶剂-非溶剂相分离法成功制备了玉米醇溶蛋白/壳聚糖复合纳米微球(NSZ/CS),运用FT-IR、SEM和TEM等对复合纳米微球进行了表征。采用罗丹明B(RB)为模型药物分子,研究了复合纳米微球的药物释放性能。与玉米醇溶蛋白纳米微球(NSZ)相比,复合纳米微球NSZ/CS对RB和Dox·HCl的包封率显著上升,分别可达83.5%和75.3%。NSZ/CS对RB的累积释放量也大幅度提高。在模拟人工胃液和人工肠液中,NSZ/CS对RB释放36 h后,累积释放量分别为85.2%和95.4%。进一步将NSZ/CS用于负载抗癌药物盐酸阿霉素(Dox·HCl),发现Dox@NSZ/CS在p H=7.4的磷酸缓冲液(PBS)中的累积释放量达91.0%。复合纳米微球NSZ/CS有望作为水溶性药物载体应用于生物医药领域。Using solvent-nonsolvent phase separation method,a kind of edible composite nanoparticles（ NSZ / CS） for delivering water-soluble drugs has been prepared by biocompatible protein（ Zein） and chitosan. The obtained NSZ / CS composite nanoparticles were characterized by FT-IR spectra,TEM and SEM. Using rhodamine B（ RB） asa model drug,release behaviors of NSZ / CS were investigated. Compared with the zein nanoparticles,the encapsulation efficiency of NSZ / CS was enhanced significantly,which got to83. 5% and 75. 3%,respectively. The cumulative release ratio of NSZ / CS on RB was enhanced obviously. After releasing 36 hrs,the release ratio of RB@ NSZ / CS in SGF and SIF achieved at 85. 2% and 95. 4%,separately. NSZ / CS was also applied to encapsulate an anti-cancer drug doxorubicin（ Dox·HCl）. It was found that the cumulative release ratio of Dox @ NSZ / CS was obviously enhanced,got to 91. 0% in PBS（ p H： 7. 4）. NSZ / CS composite nanoparticles are a promising candidate in drug delivery system.

关 键 词：玉米醇溶蛋白 壳聚糖 蛋白基药物载体 控制释放 

分 类 号：O636.12[理学—高分子化学]