加味乌梅丸诱导胰腺癌sw1990细胞NOD-SCID小鼠移植瘤细胞凋亡的实验研究  

Apoptosis Induced by Wumei Pill in Pancreatic Cancer Cell sw1990 in NOD/SCID Mice

作　　者：赵伟鹏[1] 李波[1] 黄金昶[2] 

机构地区：[1]北京中医药大学,北京100029 [2]中日友好医院,北京100029

出　　处：《中国中医急症》2015年第2期194-196,共3页Journal of Emergency in Traditional Chinese Medicine

基　　金：北京市科学技术委员会重大项目(No.D131100002213004)

摘　　要：目的观察加味乌梅丸在体内诱导移植性人胰腺癌细胞凋亡的作用。方法将20只荷瘤小鼠分为对照组、用药组各10只。NOD/SCID小鼠皮下移植瘤造模成功后,用药组每日灌胃加味乌梅丸生药0.4 g/0.4 m L,对照组每日给予无菌注射用水0.4 m L。给药期间每5日测量并计算皮下移植瘤体积,绘制移植瘤生长曲线。20 d后处死小鼠,称取瘤质量,流式细胞仪检测肿瘤细胞凋亡率,TUNEL染色标记细胞凋亡,观察细胞形态学改变。结果用药组移植瘤的体积的增长幅度明显小于对照组(P<0.05)。加味乌梅丸对NOD/SCID小鼠移植性人胰腺癌瘤抑瘤率为22%,流式细胞分析用药组的早期凋亡率为(29.6833±0.76)%,与对照组(17.0167±2.75)%相比,明显提高(P<0.01),TUNEL法检测用药组肿瘤细胞凋亡指数(A1)为(59.4±19.3)%,与对照组的(19.2±7.4)%比较明显提高(P<0.01)。结论加味乌梅丸可以通过促进肿瘤细胞的凋亡而发挥抑制肿瘤的作用。Objective： To study the effect of Wumei pill on the apoptosis of xenografts of human pancreatic cancer cell sw1990 in NOD/SCID mice. Method： 20 tumor-bearing mice were randomly divided into control group and Wumei pill group. After the murine model of human pancreatic carcinoma was established,mice in two groups received either Wumei pill decoction 0.4 g/0.4 m L/d or sterile water 0.4 m L/d for 20 days. Measurement and calculation of tumor volume in mice performed every 5 days during the 20 days. At the same time,the tumor growth curve was drawn. After treatment of 20 days,the mice were executed. The weight of tumor was evaluated.The apoptotic rates were examined by FCM and given TUNEL labeling. Morphological changes were observed by microscopy. Results： The increase of xenograft tumor volume in Wumei pill group was less than that in control group. Compared with the control group,tumor growth was significantly inhibited by Wumei pill（22%）. Apoptotic index（A1） were significantly higher（29.6833±0.76）% determined by FACScan and（59.4 19.3）% by TUNEL in Wumei pill treatment group than those in the control group [FACScan（17.0167 ±2.75）% TUNEL（19.2 7.4）% ].Conclusions： Wumei pill can inhibit pancreatic cancer by inducing pancreatic tumor cell apoptosis.

关 键 词：乌梅丸 胰腺癌 细胞凋亡 

分 类 号：R285[医药卫生—中药学]