5种克罗诺杆菌鞭毛蛋白fliC基因的克隆和序列分析  

Cloning and sequence analysis of flagellin gene fliC from five Cronobacter species

作　　者：姜华[1] 徐湾[1] 张逸飞[1] 邹小倩 裴尚飞 赵珉生 李远宏[1] 

机构地区：[1]徐州医学院公共卫生学院,江苏徐州221004

出　　处：《江苏师范大学学报（自然科学版）》2015年第1期80-83,共4页Journal of Jiangsu Normal University：Natural Science Edition

基　　金：国家自然科学青年基金资助项目(31401595);徐州医学院引进优秀人才科研启动基金项目(D2014002);江苏省高等学校大学生实践创新训练计划项目(201410313012Z)

摘　　要：根据C.sakazakii BAA 894全基因组序列中fliC基因（Gene ID：CP000783.1）,设计特异性引物,通过PCR方法扩增了5种克罗诺杆菌fliC基因的全长序列,并通过生物信息学方法对fliC基因进行了序列分析.序列分析结果表明：fliC基因的全长为837bp,编码279个氨基酸.同源性分析表明：5种克罗诺杆菌fliC基因的序列相似性为92.11%～99.40%;与其它细菌的fliC序列进行比较,其核酸序列同源性为51.73%～82.57%.这表明克罗诺杆菌fliC基因具有较高的保守性,可作为制备克罗诺杆菌多克隆抗体或单克隆抗体的一种候选抗原.To investigate the possibility of fliC as a candidate antigen for antibody production,primers were designed based on the genome sequence of C.sakazakii BAA-894（Gene ID：CP000783.1）published in GenBank.The flagellar gene fliC from five Cronobacter species was amplified by PCR.Sequence analysis revealed that fliC gene fragment is 837 bp long and encodes a putative protein of 279 amino acids.Blast searches based on fliC gene sequences showed high sequence identity（92.11%～99.40%）within the five Cronobacterspecies,and relative low sequence identity（51.73%～82.57%）with other bacteria that can be download from GenBank.The results indicated that fliC is a highly conserved protein within the genus Cronobacter,which might be a candidate antigen for the production of Cronobacter polyclonal and monoclonal antibody.

关 键 词：克罗诺杆菌 鞭毛蛋白fliC基因 序列分析 

分 类 号：R378[医药卫生—病原生物学]