壮通饮对糖尿病肾病大鼠肾皮质CTGF、TGF-β_1、PAI-1、FN细胞因子mRNA表达的影响  

Effects of Chinese herbal Zhuangtongyin on mRNA expression of CTGF,TGF-β1,PAI-1,FN cytokines in renal cortex of diabetic nephropathy rats

作　　者：江旭锋[1] 曾庆春[1] 刘军杰[1] 曾怡[2] 苏莉莉[2] 黄岑汉[2] 刘燕平[1] 

机构地区：[1]广西中医药大学,广西南宁530001 [2]右江民族医学院,广西百色533000

出　　处：《右江民族医学院学报》2015年第1期5-7,13,共4页Journal of Youjiang Medical University for Nationalities

基　　金：广西自然科学基金项目(2011GXNSFA018214);广西壮族自治区卫生厅中医药科技专项(GZKZ10-043)

摘　　要：目的实验性研究壮通饮对糖尿病肾病(DN)大鼠肾皮质结缔组织生长因子(CTGF)、转化生长因子(TGF-β1)、血浆纤溶酶原激活物抑制物(PAI-1)、纤维连接蛋白(FN)细胞因子mRNA表达的影响,并探讨壮通饮对DN大鼠肾脏的保护作用机制。方法采用单肾切除术合并腹腔注射链脲佐菌素(STZ)诱导制备DN大鼠模型,并随机分为空白对照组、假手术组、模型组、卡托普利西药组(1g/kg)和壮通饮低(6.8g/kg)、中(13.6g/kg)、高(27.2g/kg)剂量7个组。于实验给药治疗6周后,用荧光实时定量RT-PCR法检测大鼠肾皮质CTGF、TGF-β1、PAI-1、FN细胞因子mRNA的表达。结果壮通饮可以降低DN大鼠肾皮质CTGF、TGF-β1、PAI-1、FN细胞因子mRNA的表达。结论壮通饮能改善肾皮质CTGF、TGF-β1、PAI-1、FN细胞因子mRNA的表达,以减轻肾脏病理改变,对DN大鼠的肾脏具有一定的保护作用。Objective This article aims to laboratory study the effects of Chinese herbal Zhuangtongyin on mRNA expression of connective tissue growth factor（CTGF）,transforming growth factor（TGF-β1）,plasminogen activator inhibitor 1（PAI-1）,fibronectin（FN）cytokines in renal cortex of diabetic nephropathy（DN）rats,and to explore the mechanism of Zhuangtongyin in protecting the kidney of DN rats. MethodsDN rat models were induced and prepared by mono-nephrectomy combined intraperitoneal injection Streptozotocin（STZ）,and the rats were randomly divided into 7groups：a blank control group,a sham operation group,a model group,a western medicine of Captopril group,（1g/kg）and Zhuangtongyin low dose（6.8g/kg）,medium dose（13.6g/kg）and high dose（27.2g/kg）groups.Six weeks after drugs administration,a fluorescent quantitative real-time RT-PCR was used to measure the mRNA expression of CTGF,TGF-β1,PAI-1,FN cytokines in rats renal cortex. Results Zhuangtongyin could down-regulate mRNA expressions of CTGF,TGF-β1,PAI-1,FN cytokines in renal cortex of DN rats. Conclusion Zhuantgongyin can protect to a certain extent of DN rats kidney by improving he mRNA expression of CTGF,TGF-β1,PAI-1,FN cytokines in rats renal cortex for lessening the pathological changes of kidney.

关 键 词：壮通饮 糖尿病肾病 细胞因子类 荧光实时定量RT-PCR mRNA表达 

分 类 号：R285.5[医药卫生—中药学]