运动训练期间不同时间常压吸氧对小白鼠血清SOD、MDA的影响  

The effect of inhaling oxygen at different time during sport training on serum SOD,MDA in mice

作　　者：卢珍梅 杨永浩[1] 梁柳丹[1] 韦维[1] 何显教[2] 

机构地区：[1]右江民族医学院 [2]右江民族医学院生理学教研室,广西百色533000

出　　处：《右江民族医学院学报》2015年第1期8-10,共3页Journal of Youjiang Medical University for Nationalities

基　　金：2013年度广西壮族自治区级大学生创新训练计划立项项目(QJCX201333)

摘　　要：目的探讨运动训练期间不同时间常压吸氧对小白鼠血清超氧化物歧化酶(SOD)、丙二醛(MDA)含量的影响。方法 100只昆明种小白鼠,随机分为非运动训练组、运动训练不吸氧组、运动训练前吸氧组、运动训练后吸氧组、运动训练前后均吸氧组共五组,每组20只。除非运动训练组外,其余各组每天进行30 min的无负重游泳训练,在运动训练前、后不同时间给予吸入一定量的氧气或不吸氧。运动训练后第21d清晨,每组各取10只,测血清SOD、MDA含量;另外10只进行游泳力竭运动,运动后取血,测量血清SOD、MDA。结果在安静状态下运动训练后的各组血清SOD活力均高于非运动训练组(P<0.05或P<0.01),运动训练不吸氧组和运动训练前吸氧组SOD活力低于运动训练后吸氧组(P<0.01);经运动训练不吸氧组和运动训练后吸氧组,其血清MDA含量高于非运动训练组和运动训练前吸氧组(P<0.05或P<0.01)。力竭运动后,经过运动训练之后不管吸氧与否,其血清SOD活力均高于非运动训练组和运动训练前吸氧组(P<0.05或P<0.01);运动训练前吸氧组的MDA含量低于运动训练不吸氧组或运动训练后吸氧组(P<0.05或P<0.01)。除运动训练后吸氧组外,非运动训练组的MDA含量均高于其他各组(P<0.05或P<0.01)。结论运动训练期间,于运动训练前吸入一定量的氧气,可减轻自由基的损伤,延缓运动性疲劳的发生,但运动训练后吸氧可能会引起自由基损伤。Objective To observe the effect of inhaling oxygen at different time during sport training on serum superoxide dismutase（SOD）and malomalondialdehyde（MDA）in mice. Methods One hundred Kunming mice were randomly divided into five groups：no sport training group,sport training without oxygen inhalation group,oxygen inhalation before sport training group,oxygen inhalation after sport training group,oxygen inhalation before and after sport training group.Except for the no sport training group,four groups were forced to swim 30 minutes every day without weight loading,and were or not exposed to 50% oxygen before or/and after exercise training.After 20 days of training,serum MDA and SOD were measured in 50mice（10in each group）in a quiet state,another 10 mice in each group were measured after swimming exhaustion. Results In a quiet state,the SOD activities in every sport training group were higher than the no sport training group（P〈0.05 or P〈0.01）,and SOD activity in sport training without oxygen inhalation group and oxygen inhalation before sport training group were lower than that in oxygen inhalation after sport training group（P〈0.01）.The MDA amount in sport training without inhalation oxygen group and inhalation oxygen after sport training group were higher than those in no sport training group or inhalation oxygen before sport training group（P〈0.05 or P〈0.01）.After exhaustive exercise,whether inhaling oxygen or not,serum SOD activity of sport training group were higher than no sport training or inhaling oxygen before exercise（P〈0.05 or P〈0.01）;MDA content of oxygen inhalation before sport training was lower than only training without inhalation oxygen,or oxygen inhalation after sport training（P〈0.05 or P〈0.01）.Except for oxygen inhalation after sport training group,the MDA amount of no sport training group were higher than other groups（P〈0.05 or P〈0.01）. Conclusion During sport training,a certain amount of oxygen inhalation before athletic training can help r

关 键 词：运动训练 氧气 超氧化物歧化酶 丙二醛 小鼠 

分 类 号：R459.6[医药卫生—治疗学]