多发性肌炎患者FAS/FASL抗原的表达与外周血淋巴细胞亚群的相关性研究  

The association of the expressions of FAS/FASL antigens and peripheral blood lymphocyte subsets in patients with polymyositis

作　　者：揭旭日[1] 王小超[1] 黄丹[1] 李红文[1] 吕丽娜[1] 陈红[1] 左瑶 

机构地区：[1]右江民族医学院附属医院,广西百色533000

出　　处：《右江民族医学院学报》2015年第1期37-38,47,共3页Journal of Youjiang Medical University for Nationalities

基　　金：广西壮族自治区卫生厅课题(Z2014523)

摘　　要：目的分析多发性肌炎（PM）患者FAS/FASL抗原的表达与外周血淋巴细胞亚群的相关性。方法选取17例我院2012年10月～2014年10月接诊的活动期PM患者为研究对象;对照组为非骨骼肌疾病者20例。免疫组化法分别检测两组患者FAS/FASL蛋白质在不同部位的表达,流式细胞仪测定两组外周血CD3^＋、CD4^＋、CD8^＋、CD19^＋、NK细胞百分率;分析PM患者FAS/FASL抗原的表达与外周血淋巴细胞亚群的关系。结果结果 FAS、FASL抗原在PM肌纤维表面及浸润的淋巴细胞均有较强的表达,与对照组相比,差异有统计学意义（P〈0.01）;CD4^＋、CD8^＋T细胞百分比、CD4^＋/CD8^＋的比值高于对照组,差异有统计学意义（P〈0.05）。结论可能CD4^＋T细胞介导细胞免疫与FAS/FASL蛋白的促进肌细胞凋亡关系更加密切。Objective To analyze the correlation between the expression of FAS/FASL antigens and peripheral blood lymphocyte subsets in patients with polymyositis（PM）. Methods Seventeen patients with active polymyositis cared at our hospital from October 2012 to October 2014 were involved in this study,and 20 patients with non-skeletal muscle disease were chosen as the controls.An immunohistochemistry method was used to measure the expressions of FAS/FASL proteins at different loci of the two groups,respectively.A flow cytometry instrument was used to detect the peripheral blood lymphocyte subgroup CD3^＋,CD4^＋,CD8^＋,CD19^＋,and NK cells percentage.A statistical analysis was performed on the association of the expression of FAS/FASL antigens and peripheral blood lymphocyte subsets in PM patients. Results PM patients were found with higher FAS and FASL antigen expression on PM muscle fibers surface and infiltrating lymphocyte cells,comparing to the control group showed statistically significant difference（P〈0.01）.The elevated percentage of CD4^＋,CD8^＋T cells and the ratio of CD4^＋/CD8^＋were found in PM group,compared to the control group,there were statistically significant differences（P〈0.05）. Conclusion Maybe there were more close relationships between the CD4^＋T cell mediated cell immuning and FAS/FASL protein in promoting myocyte apoptosis.

关 键 词：多发性肌炎 FAS FASL 外周血 淋巴细胞亚群 

分 类 号：R685.2[医药卫生—骨科学]