利用基因组SSR分子标记对老芒麦品种(种质)鉴别和品种纯度鉴定  

Cultivars(Grmplasms) Discrimination and Seed Purity Assessment of Grass Elymus sibiricus Using Genomic SSR Markers

作　　者：雷云霆[1] 赵闫闫[1,2] 喻凤[1,2] 李媛[1,2] 窦全文[1] 

机构地区：[1]中国科学院西北高原生物研究所,青海西宁810001 [2]中国科学院大学,北京100049

出　　处：《草地学报》2015年第1期151-155,共5页Acta Agrestia Sinica

基　　金：青海省科技厅科技促进新农村建设计划项目(2013-N-515);中科院"西部之光"联合学者人才培养计划项目资助

摘　　要：利用从老芒麦（Elymus sibiricus）自身基因组中开发出来的6对多态性SSR引物,对3个老芒麦牧草品种和7个种质进行分子指纹图谱鉴别研究.结果表明：6对SSR引物在这10个材料中共检测出21个多态性SSR标记片段,每对引物可以检测到2～5个数目不等的片段,平均为3.5个.6对引物中3对核心引物组合可以将这10个材料进行有效鉴别.进一步选用3对引物对2个老芒麦品种进行纯度鉴定,表明SSR标记可以对品种群体中具有变异位点的个体进行有效鉴定,同时揭示野生栽培品种‘同德’老芒麦比育成品种‘多叶’老芒麦具有较高的遗传变异度.Molecular fingerprinting maps of 3 Elyrnus sibiricus cultivars and 7 germplasms were carried out using 6 simple sequences repeat （SSR） markers developed from the genomic DNA of E. sibiricus. A total of 21 polymorphic SSR fragments were detected in all the tested materials, and 2 to 5 alleles were detected by each pair of SSR primer with an average of 3.5. All the 3 cultivars and 7 germplasms could be well dis- tinguished by the fingerprinting maps constructed by 3 pairs of core primers. In addition, seed purity as- sessment was conducted in cultivars ＇Tongde~ and ＇Duoye＇ with 3 pairs of SSR primers. It showed that seed purity and the polymorphism of SSR alleles could be effectively identified. Moreover, it revealed that ＇Tongde＇, a domesticated cultivar, had much lower purity and higher polymorphic alleles than ＇Duoye＇, a breeding eultivar. The SSR markers used in this study can be a powerful tool for cultivars or germplasms i- dentification, seeds purity assessment in E. sibiricus.

关 键 词：老芒麦 基因组SSR分子标记 品种(种质)鉴别 纯度鉴定 

分 类 号：S324[农业科学—作物遗传育种]