金黄色葡萄球菌耐药性与消毒剂抗性基因qac(A/B)的研究  

A study on drug resistance and disinfection-resistant gene qac(A/B) in Staphylococcus aureus

作　　者：王笑笑[1] 傅鹰[2] 林军明[1] 胡国庆[1] 陆烨[1] 

机构地区：[1]浙江省疾病预防控制中心,浙江杭州310051 [2]浙江大学医学院附属邵逸夫医院

出　　处：《浙江预防医学》2015年第2期134-136,146,共4页Zhejiang Journal of Preventive Medicine

基　　金：中华医院感染控制研究基金

摘　　要：目的分析金黄色葡萄球菌耐药性和消毒剂抗性基因携带情况。方法从医院患者的痰液、分泌物、尿液和腹腔引流液等临床标本中分离金黄色葡萄球菌菌株,采用浓度梯度法(E-test)测定对常用抗菌药物的敏感性,用PCR法测定消毒剂抗性基因携带情况。结果 36株金黄色葡萄球菌对克林霉素、环丙沙星、红霉素、左旋氧氟沙星、苯唑西林和青霉素G的耐药率分别为94.44%、75.00%、94.44%、72.22%、97.22%和100.00%,ICU与非ICU病区菌株的耐药率差异无统计学意义(P>0.05),14株携带有消毒剂抗性基因qac(A/B)的阳性率为38.89%。结论临床金黄色葡萄球菌呈现多重耐药,消毒剂抗性基因携带率高。Objective To study the drug resistance and disinfection- resistant gene in Staphylococcus aureus isolates.Methods The sensitivity to 17 antibacterial agents was detected in 36 strains of Staphylococcus aureus by E- test. The disinfection- resistant genes qac（ A / B） were detected by PCR. Results The resistance rates of clindamycin,ciprofloxacin,erythromycin, levofloxacin, oxacillin and penicillin G were 94. 44%,75. 00%,94. 44%,72. 22%,97. 22% and 100. 00%,respectively. There were no significantly difference of drug resistance rates between isolates from ICU setting and non- ICU setting（ P〉0. 05）,and 14（ 38. 89%） isolates were qac（ A / B） positive. Conclusion Clinically isolated staphylococcus aureus is multi- drug- resistant and the qac（ A / B） positive rate is high.

关 键 词：金黄色葡萄球菌 消毒剂抗性 基因 

分 类 号：R378.11[医药卫生—病原生物学]