Twist和E-cadherin在卵巢型子宫内膜异位症中的表达及其意义  

The expression of Twist and E-cadherin and its clinical significance in ovarian endometriosis

作　　者：赵建珍[1] 詹宏[1] 马俊彦[1] 林俊[1] 

机构地区：[1]浙江大学医学院附属妇产科医院,浙江杭州310006

出　　处：《浙江预防医学》2015年第2期137-141,共5页Zhejiang Journal of Preventive Medicine

基　　金：国家自然科学基金项目(81370694)

摘　　要：目的探讨上皮间质转化因子Twist1、Twist2和上皮黏附分子E-cadherin在卵巢型子宫内膜异位症中的表达及其意义。方法采用SP免疫组织化学法检测24例正常内膜(正常对照组)和卵巢型子宫内膜异位症异位内膜I-II期24例及III-IV期30例(异位组)的Twist1、Twist2及E-cadherin蛋白的表达染色评分分值情况,并作不同组间的比较。结果 Twist1、Twist2染色评分异位组I-II期(4.25±0.79,3.83±0.96)及IIIIV期(4.67±0.88,4.30±0.95)均高于正常组(3.50±0.83,3.54±0.88)(P均<0.05);Twist2染色评分异位组III-IV期(4.30±0.95)高于I-II期(3.83±0.96)(P<0.05)。E-cadherin蛋白染色评分异位组I-II期(3.79±0.98),III-IV期(3.57±0.73)均低于正常组(4.54±0.93)(P均<0.05)。Twist1和Twist2分别与E-cadherin呈负相关(r1=-0.42,r2=-0.81,P均<0.05);Twist1和Twist2呈正相关(r=0.32,P<0.05)。结论 Twist1、Twist2、E-cadherin表达与卵巢型子宫内膜异位症的发生相关,子宫内膜异位细胞侵袭转移能力的增强可能与Twist1、Twist2的高表达和E-cadherin的低表达有关。Objective To know the expression and clinical significance of Twist1,Twist2 and E- cadherin in ovarian endometrium. Methods Immunohistochemistry was used to detect the expression of Twist1,Twist2 and E- cadherin in tissues of ovarian endometrium（ EMs I- II,n = 24; EMs III- IV,n = 30） andnormal endometriums without endometriosis（ Control,n = 24）. Results The expression levels of Twist1,Twist2 were significantly higher in EMs I- II（ 4. 25 ± 0. 79,3. 83 ± 0. 96 respectively） and EMs III- IV（ 4. 67 ± 0. 88,4. 30 ± 0. 95 respectively） while the values of the control group were 3. 50 ± 0. 83,3. 54 ± 0. 88 respectively（ both P〈0. 05）,and the group of EMs III- IV had a higher expression of Twist2（ 4. 30 ± 0. 95） than that in EMs I- II（ 3. 83 ± 0. 96）（ P〈0. 05）. At the same time,the expression of E-cadherin were significantly lower in EMs I- II（ 3. 79 ± 0. 98） and EMs III- IV（ 3. 57 ± 0. 73） than the control（ 4. 54 ±0. 93）（ P〈0. 05）. The expression of Twist1 and Twist2 was negatively related to that of E- cadherin respectively（ both P〈0. 05）,while the expression of Twist1 was positively related to that of Twist2（ P〈0. 05）. Conclusion Twist1,Twist2 and E- cadherin are closely related to the prevalence of endometriosis,while the ability of invasion and metastasis may be associated with the high expression of Twist1 and Twist2,as well as the low expression of E- cadherin.

关 键 词：子宫内膜异位症 TWIST1 Twist2 E-CADHERIN 上皮细胞间质化转变 

分 类 号：R711.71[医药卫生—妇产科学]