姜黄素对大鼠慢性脑缺血诱发脑损伤的保护作用及分子机制研究  

Protective Effects of Curcumin on Brain Injury Induced by Chronic Cerebral Ischemia in Rats and Its Molecular Mechanism

作　　者：杨柳[1] 张敏[1] 贺曦[1] 朱洁[1] 王进平[1] 孟涛[1] 

机构地区：[1]重庆市急救医疗中心神经内科,重庆400014

出　　处：《中国药业》2015年第4期31-34,共4页China Pharmaceuticals

摘　　要：目的观察姜黄脑素对缺血后海马组织中细胞色素C以及caspase-3蛋白的表达情况,探讨姜黄素发挥其神经保护作用的机制。方法将50只SD大鼠随机分为假手术组(Sham)、正常对照组、缺血后二甲基亚砜(DMSO)干预对照组、姜黄素高剂量组(100 mg/kg)和姜黄素低剂量组(50 mg/kg)。所有治疗组于术后24 h开始以姜黄素每日腹腔注射,连续28 d。给药结束后,对各组进行Morris水迷宫试验测试大鼠空间学习和记忆能力,然后处死大鼠,用HE染色和尼氏染色观察海马组织的病理形态变化,Western-Blot法检测海马组织中细胞色素C和capase-3蛋白的表达情况。结果与各对照组比较,姜黄素明显增强了慢性脑缺血大鼠的空间学习和记忆能力,而且明显减轻了神经细胞的凋亡。Western-Blot法检测到姜黄素使海马组织中细胞色素C和caspase-3蛋白表达水平明显降低(P<0.05)。结论姜黄素对慢性大脑缺血诱发脑损伤有明显的神经保护作用,其机制可能是通过细胞色素C途径抑制caspase-3蛋白的表达而发挥作用。Objective To observe the effects of curcumin on the expression of cytochrome C and caspase- 3 protein in the hippocampus tissue after ischemia,and to explore the potential mechanism for curcumin playing its neuroprotection role. Methods 50 Sprague- Dawley rats were randomly divided into the sham- operation group（ Sham）,normal control group,bilateral common carotid occlusion（ 2VO） ＋dimethyl sulfoxide（ DMSO） intervention control group,curcumin high dose group（ 100 mg / kg） and curcumin low dose group（ 50 mg / kg）.All treatment groups were intraperitoneally injected by curcumin with in 24 h after operation for consecutive 28 d. Each group was performed the Morris water maze test for detecting the spatial learning and memory ability after medication. Then the rats were killed and the pathological morphological changes of hippocampus were observed by the hematoxylin and eosin（ HE） staining and Nissl staining. Western- Blot was used to detect the expression of cytochrome C and caspase- 3 in the hippocampus tissue. Results Compared to the various control groups,curcumin significantly enhanced the spatial learning and memory ability in the chronic ischemic rats and obviously alleviated the apoptosis of nerve cells. The Western- Blot detection showed that curcumin significantly decreased the expression levels of cytochrome C and caspase- 3 protein in the hippocampus tissue（ P〈 0. 05）. Conclusion Curcumin has obviously neuroprotective effect on the brain injury induced by chronic cerebral ischemia,its mechanism could be that curcumin might inhibit caspase- 3protein expression through cytochrome C pathway.

关 键 词：姜黄素 慢性脑缺血 细胞色素C 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学] R282.71[医药卫生—中医学]