正交试验优选酸枣仁总皂苷提取工艺  

Optimization of Extraction Technology for Total Saponins from Ziziphi Spinosae Semen by Orthogonal Test

作　　者：林海成 祝洪艳[1] 何忠梅[1] 王国丽[1] 张连学 

机构地区：[1]吉林农业大学中药材学院,长春130118 [2]吉林省人参工程技术创新中心,长春130118

出　　处：《中国实验方剂学杂志》2015年第5期42-45,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技支撑计划项目(2011BAI03B010602);国家"重大新药创制"科技重大专项(2012ZX09304006);国家公益性行业(农业)科研专项(201303111)

摘　　要：目的：优选酸枣仁总皂苷的提取工艺,为该成分的工业化生产提供参考。方法：采用HPLC-UV检测酸枣仁皂苷A和B的含量,流动相乙腈-水（34∶66）,检测波长204 nm。在单因素试验基础上,通过正交试验考察乙醇体积分数、乙醇用量、提取时间及提取次数对酸枣仁总皂苷提取工艺的影响。结果：酸枣仁皂苷A,B的线性范围分别为0.24-2.40,0.12-1.20μg,平均加样回收率分别为100.39%,99.40%。酸枣仁总皂苷最佳提取工艺为加12倍量70%乙醇回流提取2次,每次1.5 h;酸枣仁皂苷A,B的平均质量分数分别为0.606,0.228 mg·g^-1。结论：建立的HPLC检测酸枣仁皂苷A,B含量方法准确可靠,优选的酸枣仁总皂苷提取工艺稳定可行,具有节约成本、省时等特点,适用于工业化生产。Objective： To optimize extraction technology of total saponins from Ziziphi Spinosae Semen and provide a reference for industrial production of this component. Method： HPLC-UV method was employed to detect contents of jujuboside A and jujuboside B with mobile phase of aeetonitrile-water （34： 66） and detection wavelength at 204 nm. On the basis of single factor tests, effects of ethanol concentration, liquid-sodid ratio, extraction time and extraction times on extraction process were investigated by orthogonal test. Result： Linear ranges of jujuboside A and jujuboside B were 0.24-2.40, 0.12-1.20 μg, average recovery of them were 100. 39% and 99.40%. The best extraction technology of total saponins from Ziziphi Spinosae Semen was as following： extracted 2 times with 12 times the amount of 70% ethanol for 1.5 h per time; average contents of jujuboside A and jujuboside B were 0. 606, 0. 228 mg -g-I, respectively. Conclusion： This established HPLC method is accurate and reliable to detect contents of jujuboside A and jujuboside B. At the same time, this optimized extraction technology of total saponins from Ziziphi Spinosae Semen is stable and feasible with advantages of energy-saving, time-saving and so on, so it is suitable for industrial production.

关 键 词：酸枣仁 酸枣仁皂苷A 酸枣仁皂苷B 总皂苷 

分 类 号：R283.6[医药卫生—中药学] R284.2[医药卫生—中医学]