HPLC同时测定百令胶囊中5种核苷类成分的含量  

Simultaneous Determination of Five Nucleosides in Bailing Capsule by HPLC

作　　者：郑瑜倩 程巧鸳 祝明 张昀 

机构地区：[1]浙江中医药大学,杭州310053 [2]浙江省食品药品检验研究院,杭州310004 [3]中美华东制药有限公司,杭州310011

出　　处：《中国实验方剂学杂志》2015年第5期61-64,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"重大新药创制"科技重大专项(2012ZX09304-005)

摘　　要：目的：建立HPLC同时测定百令胶囊中5种核苷类成分尿苷酸、尿苷、腺苷酸、鸟苷和腺苷含量的方法。方法：采用Ultimate AQ C18色谱柱（4.6 mm×250 mm,5μm）,以甲醇-0.05 mol·L-1磷酸二氢钾溶液为流动相进行梯度洗脱,流速1.0m L·min-1,检测波260 nm,柱温25℃。结果：在该色谱条件下,尿苷酸、尿苷、腺苷酸、鸟苷和腺苷分离良好,且分别在35.25-528.8（r=1.000）,38.07-571.1（r=1.000）,34.96-524.5（r=0.999 8）,38.34-575.1（r=1.000）,39.84-597.6 ng（r=1.000）线性关系良好。加样回收率分别为101.2%,103.1%,96.7%,101.4%,103.2%,RSD分别为1.9%,1.1%,1.3%,2.7%,1.6%。结论：该HPLC含量测定方法结果准确、可靠,具有良好的专属性、精密度、重复性和稳定性,可用于百令胶囊的质量控制。Objective ： To establish an HPLC method for simultaneous determination of five nucleosides, uridine 5＇-monophosphate, uridine, adenosine 5＇-monophosphate monohydrate, guanosine, adenosine in Bailing capsules. Method： The separation was developed on an Ultimate AQ C18 column （4.6 mm ×250 mm,5μm） by gradient elution with methanol-0.05 mol·L^-1 potassium dihydrogen phosphate solution, with flow rate at 1.0 mL·min-1 and column temperature at 25 ℃. The detection wavelength was at 260 nm. Result： The baseline separation of uridine 5＇-monophosphate, uridine, adenosine 5＇-monophosphate monohydrate, guanosine, and adenosine was successfully achieved. The results showed that these five ingredients had good linearity within the test ranges of 35.25-528.8 （r =1.000）, 38.07-571.1 （r =1.000）, 34.96-524.5 （r =0.999 8）, 38.34-575. 1 （r=1.000） and 39.84-597.6 ng （r = 1.000）, respectively. The recovery were 101.2% （RSD 1.9%）, 103. 1% （RSD 1. life）, 96.7% （RSD 1.3%）, 101.4% （RSD 2.7%） and 103.2% （RSD 1.6%）, respectively. Conclusion： The developed method of the HPLC-fingerprint and quantitative analysis showed good specificity, precision, reproducibility, stability and could be used for the quality control of Bailing capsules.

关 键 词：百令胶囊 高效液相色谱法 尿苷酸 尿苷 腺苷酸 鸟苷 腺苷 

分 类 号：R284.1[医药卫生—中药学]