HPLC-UV-ELSD同时测定散结镇痛胶囊中5个指标成分含量  

Simultaneous Determination of Five Compounds in Sanjie Zhentong Capsules by High-performance Liquid Chromatography Coupled with Ultraviolet and Evaporative Light Scattering Detectors

作　　者：张广仁[1,2] 吴云[2,3] 吴莎[2,4] 萧伟[1,2,3] 

机构地区：[1]南京中医药大学,南京210000 [2]中药制药过程新技术国家重点实验室,江苏连云港222001 [3]江苏康缘药业股份有限公司,江苏连云港222001 [4]北京中医药大学,北京100020

出　　处：《中国实验方剂学杂志》2015年第5期65-68,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"重大新药创制"科技重大专项(2011ZX09201-201-20)

摘　　要：目的：建立HPLC-UV-ELSD同时测定散结镇痛胶囊中皂苷类和黄酮类成分的方法,比较15批散结镇痛胶囊中各成分含量。方法：采用HPLC-UV-ELSD联用法,Waters Symmetry@C18色谱柱（4.6 mm×250 mm,5μm）,乙腈（A）-水（B）梯度洗脱（1-13 min,19%-37%A;13-50 min,37%-36%A）,流速1 m L·min^-1,检测波长278 nm;蒸发光散射检测器（ELSD）漂移管温度45℃,载气流速3.7 L·min^-1,同时测定散结镇痛胶囊中三七皂苷R1,人参皂苷Rg1,人参皂苷Rb1,龙血素A和龙血素B的含量。结果：建立了同时测定散结镇痛胶囊中3个皂苷类和2个黄酮类成分含量的方法,线性关系良好（0.999 5-0.999 7）,平均回收率为99.1%-104.2%。结论：该方法简便,重复性好,适用于散结镇痛胶囊皂苷类和黄酮类成分的同时测定,亦适用于散结镇痛胶囊的的质量控制。Objective： To establish a quantitative method of three main saponins and two major active flavonoids in Sanjie Zhentong capsules by high-performance liquid chromatography coupled with ultraviolet detectors and evaporative light scattering detectors （HPLC-UV-ELSD） , and compare the contents of notogin-senoside R1 , ginsenoside RgI , ginsenoside RhI , loureirin A, loureirin B among 15 batches of the drugs. Method： HPLC-UV- ELSD was conducted on a Waters Symmetry C18 （4.6 mm ×250 mm,5μm） with gradient elution, the mobile phase was ACN and H20, the temperature of drift tube was 45 ℃ and the nebulizing gas flow rate was 3.7 L·min^-1 Result： Five compounds were simultaneously detected with one injection, good linear relationships were found （0. 999 5-0. 999 7） , and the average recovery rates were 99.1%-104.2% method is simple and reproducible, which can be used for quality control of Conclusion： The HPLC-UV-ELSD Sanjie Zhentong capsules

关 键 词：散结镇痛胶囊 高效液相色谱-紫外-蒸发光散射 三七皂苷R1 人参皂苷Rg1 人参皂苷Rb1 龙血素A 龙血素B 

分 类 号：R284.1[医药卫生—中药学]