HPLC测定复方黄葵颗粒中8种成分  

Simultaneous Determination of Eight Components in Compound Huangkui Granules by HPLC

作　　者：江静怡 刘欢[1] 王丽灵[1] 刘志辉[2] 

机构地区：[1]南京中医药大学,南京210046 [2]南京中医药大学附属医院,南京210029

出　　处：《中国实验方剂学杂志》2015年第5期95-98,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：江苏省药学会-奥赛康医院药学基金项目(201302);江苏省科技支撑计划项目(BE2012777)

摘　　要：目的：建立HPLC同时测定复方黄葵颗粒中毛蕊异黄酮葡萄糖苷、金丝桃苷、芦丁、异槲皮苷、杨梅素、槲皮素、马钱苷、莫诺苷的方法。方法：采用Aglient C18色谱柱（4.6 mm×250 mm,5μm）,以乙腈-0.05%甲酸水溶液为流动相进行梯度洗脱,流速0.8 m L·min^-1,检测波长分别为240,260,360 nm,柱温30℃。结果：毛蕊异黄酮葡萄糖苷、金丝桃苷、芦丁、异槲皮苷、杨梅素、槲皮素、莫诺苷、马钱苷线性范围分别为3.5-35.0（r=0.999 6）,32.09-320.9（r=0.999 7）,2.21-22.12（r=0.999 7）,18.68-186.9（r=0.999 8）,6.32-63.20（r=0.999 7）,3.54-35.44（r=0.999 6）,12.39-123.9（r=0.999 6）,12.88-128.8 mg·L^-1（r=0.999 6）。平均回收率分别为98.1%（RSD 1.7%）,99.7%（RSD 0.6%）,98.7%（RSD 2.5%）,100.1%（RSD 1.4%）,99.7%（RSD 1.0%）,99.9%（RSD 2.7%）,99.0%（RSD 1.2%）,99.7%（RSD 1.3%）。结论：所建立方法简便、准确,重复性好,可作为复方黄葵颗粒的质量控制。Objective： To establish an HPLC method for simultaneously determining the contents of calycosin-7-O-β-D-glucoside, hypericin, rutin, isoquercetin, myricetin, quercetin, loganin, morroniide in compound Huangkui granules. Method： The determination was performed on an Aglient Cls （4.6 mm×250 mm,5μm） chromatographic column, the mobile phase was acetonitrile-0.05% formic acid aqueous solution with gradient elution at a flow rate of 1.0 mL ·min^-1 ; the detection wavelength was 240, 260 and 360 nm, column temperature was 30 ℃. Result： The calibration curves of calycosin-7-O-β-D-glucoside, hypericin, rutin, isoquercetin, myricetin, quercetin, loganin, morroniide were linear in the ranges of 3.5-35.0 （ r = 0. 999 6） , 32.09-300.9 （r=0.999 7）, 2.21-22.12 （r=0.999 7）, 18.68-186.9 （r=0.999 8）, 6.32-63.20 （r= 0.999 7）, 3.54-35.44 （r=0.999 6）, 12.39-123.9 （r=0.999 6）, 12.88-128.8 mg·L^-1 （r=0.999 6）, respectively. The average recoveries were 98. 1% （ RSD 1.7% ）, 99. 7% （ RSD 0. 6% ）, 98.7% （ RSD 2.5%）, 100.1% （RSD 1.4%）, 99.7% （RSD 1.0%）, 99.9% （RSD2.7%）, 99.0% （RSD 1.2%）, 99.7% （RSD 1.3% ） , respectively. Conclusion： The method established is simple, accurate and reproducible, which can be used for the quality control of Huangkui granules.

关 键 词：复方黄葵颗粒 黄芪 黄蜀葵花 酒萸肉 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]