菌株CHBT-1721与中国其他温泉嗜热菌的系统进化分析(英文)  

Phylogenetic analysis of strain CHBT-1721 with other thermophile bacteria isolated from hot springs of China

作　　者：方舒[1] 代冲[1] 晏娟[1] 

机构地区：[1]巢湖学院化学化工与生命科学学院,安徽巢湖238000

出　　处：《浙江大学学报（农业与生命科学版）》2015年第1期15-24,共10页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：Project supported by the Scientific Research Program of Chaohu University(No.XLY-201208);the Natural Science Research Program of the Higher Education Institutions of Anhui Province(No.KJ2012Z270)

摘　　要：通过对从安徽巢湖半汤温泉水中筛选到的首个嗜热菌株CHBT-1721进行初步系统进化研究,为当地政府开发利用温泉水资源以及生物多样性保护提供基础资料和科学依据。采取形态学观察、生理生化测定和16S rRNA基因序列同源性分析相结合的方法,确定该菌株的分类地位。另外,从GenBank数据库下载到22种具有学名且首次发现于中国温泉的嗜热菌的16SrRNA基因序列,通过构建系统发育树、比对16SrRNA基因序列、分析16SrRNA二级结构来研究菌株CHBT-1721在其他22种嗜热菌中的系统分布。结果表明菌株CHBT-1721属于芽孢杆菌属(Bacillus sp.)。系统发育树显示,菌株CHBT-1721与3株嗜热菌(Anoxybacillus vitaminiphilus3nP4T,A.eryuanensis E-112T和A.tengchongensis T-11T)共同形成一个进化分支,且与A.vitaminiphilus 3nP4T具有最近的亲缘关系(94%的相似度)。此外,对同一进化分支上菌株的16SrRNA二级结构分析结果显示:菌株CHBT-1721在16SrRNA二级结构的18号茎上有一个唯一的结构;与另外3株菌在12号与18号茎的16SrRNA基因序列一级结构上完全相同,但它们在二级结构上却不完全相同。总之,本文揭示了菌株CHBT-1721在中国温泉嗜热菌中所处的系统分布地位。此外,半汤温泉水与四川省普格温泉水中的化学、生物参数有一些相似之处。Summary Thermophilic bacterium, strain CHBT-1721 is the first thermophilic microorganism isolated from the hot spring of Bantang in Chaohu of Anhui Province and the phylogenetic relationship of this strain with other thermophile bacteria isolated from hot springs of China was analyzed preliminarily, which provided local government with primary data and scientific basis for utilization of Bantang hot spring water and protection of biodiversity. The strain CHBT-1721 was classified based on morphological features, physiology and biochemistry determination, and sequencing of its 16S rRNA gene. The 16S rRNA gene sequences of all the thermophilic bacteria with valid published names isolated from hot springs of China （22 thermophilic bacteria） were downloaded from the GenBank. Compared with the other 22 thermophilic bacteria, the phylogenetic diversity of strain CHBT-1721 was studied by constructing the phylogcnetic tree, aligning their 16S rRNA gene sequences and analyzing their 16S rRNA secondary structures. The strain CHBT-1721 was classified to the genus of Bacillus sp. Phylogenetic analysis showed that the strain CHBT-1721 formed a cluster in the phylogenetic tree and had 94% similarity with Anoxybacillus vitaminiphilus 3nP4T in the phylogroup. Anoxybacillus vitaminiphilus 3nP4T, A. eryuanensis E-112T and A. tengchongensis T-11r （Anoxybacillus spp. ） were also in the cluster. 16S rRNA secondary structures comparison in the same cluster also indicated that the strain CHBT-1721 had a unique 16S rRNA secondary structure characteristic in helix 18. The strain CHBT-1721 and Anoxybacillus spp. had identical 16S rRNA gene sequences in helices 12 and 18, but not all of them had identical 16S rRNA secondary structures. This current study reveals the phylogcnetic diversity of strain CHBT-1721 compared with the other 22 thermophilic bacterial We also report that the chemical and biological parameters of the hot spring water in Bantang are similar to those in Pnge of Sichuan Province.

关 键 词：嗜热菌 系统发育树 16S rRNA二级结构 茎 

分 类 号：Q939.1[生物学—微生物学]