对影响香蕉叶斑病菌喙突脐蠕孢生长和产孢碳、氮源的统计学分析  

Statistical analysis of carbon and nitrogen sources for growth and sporulation of Exserohilum rostratum causing banana leaf spot disease

作　　者：杜瑞卿[1] 林善海[2,3] 黄思良[1] 张征田[1] 覃丽萍[4] 黎起秦[3] 

机构地区：[1]南阳师范学院生命科学与技术学院,河南南阳473061 [2]广西农业科学院甘蔗研究所,南宁530007 [3]广西大学农学院,南宁530005 [4]广西农业科学院微生物研究所,南宁530007

出　　处：《浙江大学学报（农业与生命科学版）》2015年第1期64-74,共11页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：河南省高校科技创新团队支持计划项目(2010JRTSTHNO12);广西自然科学基金资助项目(桂科青2013jjBA30044);广西农业科学院科技发展基金重点项目(桂农科2013JZ12)

摘　　要：为了解不同碳源和氮源对香蕉叶斑病菌喙突脐蠕孢不同菌株的生长和产孢影响存在的共性与个性差异,该文利用聚类分析、判别分析和相关分析等多种统计分析法对实验数据进行深度解析。分析结果显示,碳源对菌株CLER09、D087与JL05的生长和产孢量有显著影响,其中乳糖是供试3菌株最适生长和产孢的碳源;在不同碳源中供试3菌株的菌落直径和产孢量之间呈极显著相关。氮源对菌株CLER09、D087与JL05的产孢量有显著影响,对菌落直径无显著影响,其中L-半胱氨酸与L-苯丙氨酸是供试3菌株生长和产孢的最适宜氮源。同时,各菌株对碳源和氮源又表现出各自的特性。因此,对于适宜香蕉叶斑病菌喙突脐蠕孢生长和产孢的碳源和氮源的选取,多菌株主要考虑其共性碳源和氮源,单菌株则应考虑菌株的各自特性碳源和氮源。Du Ruiqing , Lin Shanhai , Huang Siliang , Zhang Zhengtian , Qin Liping , Li Qiqin （ 1. School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, Henan, China; 2. Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China; 3. College of Agriculture, Guangxi University, Nanning 530005, China; 4. Microbiology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China）Summary Exserohilum rostraturn is one of the important pathogens causing banana leaf spots, which has a wide host range. The effects of carbon and nitrogen sources on colony diameters and sporulation among different isolates of E. rostratum have been analyzed using conventional variance analysis method; however, this method was impossible to differentiate the common carbon and nitrogen sources from the species and the isolate-specific ones for growth and sporulation. In-depth difference analyses of various carbon and nitrogen sources were performed to determine the nutritional characteristics of E. rostratum causing banana leaf spot disease, and to provide a basis for disease management. Three isolates （CLER09, D087 and JL05） of the pathogen were used as the experimental ones~ The Czapek＇s medium was used as a basal medium for nutritional tests on carbon and nitrogen sources. The sucrose in the basal medium was substituted with an equal amount of each of the 20 carbon sources tested. The potassium nitrate in the basai medium was substituted with an equal amount of each of the 26 nitrogen sources tested. The basal medium lacking sucrose and that lacking potassium nitrate were used as the controls for carbon and nitrogen utilization tests, respectively. The three isolates were inoculated on the basal media containing different carbon and nitrogen sources at 28 ~C for 4 days. The colony diameters and the numbers of conidia produced were separately investigated. The data obtained were evaluated using multiple statistical methods including cluster analysis

关 键 词：喙突脐蠕孢 碳源 氮源 聚类分析 判别分析 相关分析 

分 类 号：S432.4[农业科学—植物病理学]