穿山龙超高效液相色谱特征指纹图谱的研究  

UPLC characteristic chromatographic profile of Dioscoreae Nipponicae Rhizoma

作　　者：郑雅楠[1] 廖茂梁[1] 孙卫[1] 任为之 

机构地区：[1]天津药物研究院有限公司,天津300193 [2]天津世纪天龙药业有限公司,天津301741

出　　处：《现代药物与临床》2015年第1期28-32,共5页Drugs & Clinic

基　　金：天津市科技计划项目(12ZCDZSY11600;14ZCZDSY00005);天津市应用基础与前沿技术研究计划项目(14JCQNJC13800)

摘　　要：目的建立穿山龙药材的超高效液相色谱(UPLC)特征指纹图谱分析方法。方法采用Agilent Poroshell 120Bonus-RP色谱柱(50 mm×3.0 mm,2.7μm);流动相:乙腈–水,梯度洗脱;体积流量:0.8 m L/min;检测波长:203 nm;柱温:25℃;进样量:10μL。应用相似度分析软件建立穿山龙药材指纹图谱的共有模式,并对色谱峰进行指认。结果建立了穿山龙药材的UPLC特征指纹图谱共有模式,标定了14个共有峰,指认了其中3个共有峰,10批穿山龙药材的相似度为0.9以上。通过聚类分析,将13批穿山龙药材聚为4类。结论该方法快速,可用于评价穿山龙药材质量。Objective To establish a UPLC method for determination the characteristic chromatographic profiles of Dioscoreae Nipponicae Rhizoma. Methods The UPLC characteristic chromatographic profiles was carried on Agilent Poroshell 120 Bonus-RP column（50 mm × 3.0 mm, 2.7 μm） with acetonitrile - water as mobile phase in gradient elution. The detective wavelength was set at 203 nm. The temperature of column was set at 25 ℃. The flow rate was 0.8 mL/min with injection volume of 10 μL. The UPLC characteristic chromatographic profiles of Dioscoreae Nipponicae Rhizoma were established with similarity analyses and the peaks were identified. Results The common mode of the UPLC characteristic chromatographic profile was set up. There were 14 common peaks, and three of them were identified. The similarities of 10 samples to the common mode were above 0.9. The 13 batches of samples were classified into four clusters by cluster analysis. Conclusion The method is rapid, and can be used for the quality control for Dioscoreae Nipponicae Rhizoma.

关 键 词：穿山龙 超高效液相色谱 特征指纹图谱 相似度 

分 类 号：R286.02[医药卫生—中药学]