七氟烷对不同缺血时间大脑中动脉闭塞模型的影响  

The effect of different ischemic time on sevoflurane postconditioning in rat middle cerebral artery occlusion model

作　　者：王俊宽[1] 周辉[1] 陈若琨[1] 魏新亭[1] 刘献志[1] 

机构地区：[1]郑州大学第一附属医院神经外科,450052

出　　处：《中华实验外科杂志》2015年第2期356-358,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81201990）

摘　　要：目的 观察在大脑中动脉闭塞（MCAO）模型中不同缺血时间对实验结果的影响.方法 雄性SD大鼠随机分为缺血60、90和120 min 3组,在再灌注开始时吸入1.0最小肺泡浓度（MAC）七氟烷进行后处理.再灌注24h后用神经功能缺陷评分评价神经功能,氯化三苯基四氮唑（TTC）染色后计算脑梗死容积.结果 60、90和120 min缺血导致的脑梗死容积分别为26.35％、42.65％和50.02％.在90 min组,七氟烷后处理明显改善了神经功能,七氟烷组和对照组神经功能缺陷评分均数分别为1.41和2.22分,并且减小了脑梗死容积,梗死容积百分比分别为30.17％和42.65％,60和120 min组比较差异无统计学意义（P＞0.05）.结论 选择恰当的缺血时间是MCAO模型动物实验取得良好实验结果的关键.Objective To evaluate the effect of different ischemic time on sevoflurane postconditioning in rat middle cerebral artery occlusion model （MCAO）.Methods Male SD rats were randomly allocated into 3 groups subjected to MCAO for 60 min,90 min or 120 min separately and then treated with sevoflurane at the beginning of reperfusion.The infarct volume and neurological deficit scores were evaluated at 24 hours.Results The infarct volume of 60,90 and 120 min group is 26.35%,42.65% and 50.02%.In 90 min group,postconditioning with sevoflurane deceased neurological deficit scores （1.41 vs.2.22） and infarct volume （30.17% vs.42.65%）,while no significant differences were observed in 60 min and 120 min groups.Conclusion Optimal ischemic time is very important in experiment with MCAO.

关 键 词：中动脉闭塞模型 七氟烷 后处理 缺血时间 

分 类 号：R96[医药卫生—药理学]